Pesticide exposure may cause genotoxic effects by inducing the formation of micronucleus (Mn). Mn are fragments of chromosomes that remains after cells division. The increase in Mn may increase the risk of cancer formation. Our study aimed to determine the effects of lifestyle and pesticide exposure on the formation of Mn in epithelial cells from buccal swabs among paddy farmers in Malaysia. About 40 farmers who were exposed to pesticides were chosen as subjects and 30 personnels whose not directly exposed to pesticides, were chosen as the control group. Demographic and anthropometric data were obtained from questionnaires developed. Analysis of Mn formation was done using Giemsa staining (10% v/v) and the frequency of Mn formation was scored from 1000 cells per sample. Kruskal-Wallis test done between Mn frequency with age group showed a significant increase (p < 0.05) in Mn frequency in farmers as compared to the control in the age group of 30-39 , 40-49 years, and 50-59 years. Significant increased (p < 0.05) were observed between Mn frequency groups of normal BMI, pre-obese, and grade 1 obese as compared to control. Significant increase of Mn frequency (p < 0.01) was also seen among smokers and farmer’s group (15.39 ± 3.34) as compared to controls (4.76 ± 1.26). The maximum numbers of Mn found in farmers are 7 Mn per cell whereas for control group is only 3 Mn. However, most farmers had only 1 Mn (81.75 ± 6.42%) and 2 Mn (15.28 ± 5.14%). Mn frequency with the duration of exposure to pesticides in a month and the use of PPE revealed no significant difference (p = 0.27). In conclusion, the increased frequency of Mn was influenced by age, gender, BMI and smoking status of farmers besides commonly repeated duration of exposures and the use of PPE. Further studies are needed to analyze the causes of an increased in Mn among farmers.
Sekolah tahfiz merupakan institusi yang mengkhususkan pendidikan kepada pelajar yang boleh menghafaz serta mengingati keseluruhan al-Quran. Aktiviti menghafaz al-Quran dengan teknik pengucapan berulang kali akan membantu meningkatkan keupayaan otak untuk memproses, mengingati maklumat dan membina memori. Kehadiran logam berat akan memberi kesan kepada sistem saraf serta mengganggu fungsi sistem saraf pusat dan periferi di mana akan seterusnya menyebabkan berlakunya kecelaan fungsi mental dan kognitif. Keupayaan untuk belajar, mengingati atau menghafaz, penggunaan bahasa dan untuk memahami sesuatu akan terganggu serta mengurangkan IQ dan perhatian. Kajian keratan rentas telah dilakukan bagi mengukur dan mengenal pasti hubungan di antara tahap logam berat, hafazan al-Quran dan tahap kecerdasan (IQ) dalam kalangan pelajar di sekolah tahfiz berbanding sekolah bukan tahfiz terpilih di Selangor. Kepekatan logam berat ditentukan melalui analisa sampel kuku dan rambut pelajar dengan menggunakan Inductively Coupled Plasma Mass Spectrometry (ICP-MS). Ujian kecerdasan Wechsler Adult Intelligence Scale Edisi Kedua (WASI-II) digunakan untuk mengukur IQ pelajar. Borang soal selidik yang digunakan untuk mengumpulkan data demografik dianalisa menggunakan SPSS versi 23.0. Berdasarkan ujian korelasi Pearson yang dilakukan, terdapat korelasi negatif yang sangat lemah tetapi signifikan antara logam mangan dalam sampel rambut dengan tahap hafazan al-Quran (r = -0.178, p = 0.017). Selain itu, terdapat korelasi positif yang lemah tetapi signifikan antara IQ dengan tahap hafazan al-Quran (r = 0.375, p = < 0.001). Kesimpulannya, semakin tinggi kepekatan logam mangan akan menyebabkan penurunan tahap hafazan al-Quran serta apabila semakin tinggi tahap hafazan al-Quran akan menyebabkan tahap kecerdasan (IQ) juga semakin meningkat.