Displaying all 2 publications

Abstract:
Sort:
  1. Sivasothy Y, Liew SY, Othman MA, Abdul Wahab SM, Hariono M, Mohd Nawi MS, et al.
    Trop Biomed, 2021 Jun 01;38(2):79-84.
    PMID: 33973577 DOI: 10.47665/tb.38.2.044
    The NS2B/NS3 protease is crucial for the pathogenesis of the DENV. Therefore, the inhibition of this protease is considered to be the key strategy for the development of new antiviral drugs. In the present study, malabaricones C (3) and E (4), acylphenols from the fruits of Myristica cinnamomea King, have been respectively identified as moderate (27.33 ± 5.45 μM) and potent (7.55 ± 1.64 μM) DENV-2 NS2B/NS3 protease inhibitors, thus making this the first report on the DENV-2 NS2B/NS3 protease inhibitory activity of acylphenols. Based on the molecular docking studies, compounds 3 and 4 both have π-π interactions with Tyr161. While compound 3 has hydrogen bonding interactions with Gly151, Gly153 and Tyr161, compound 4 however, forms hydrogen bonds with Ser135, Asp129, Phe130 and Ile86 instead. The results from the present study suggests that malabaricones C (3) and E (4) could be employed as lead compounds for the development of new dengue antivirals from natural origin.
  2. Abdullah MS, Mohd Hairon S, Mohd Yusoff SS, Abdullah Zakaria MA, Mohd Nawi MS
    Malays J Med Sci, 2026 Apr;33(2):158-171.
    PMID: 42953854 DOI: 10.21315/mjms-01-2026-064
    BACKGROUND: Primary healthcare workers are essential in managing infectious diseases, including mpox. A validated instrument is required to assess their knowledge and readiness for mpox management. This study evaluated the content and face validity of a Malay-language mpox questionnaire for primary healthcare workers in Malaysia.

    METHODS: Questionnaire development was conducted in two stages: i) item generation and ii) judgement and quantification. Ten experts from relevant disciplines assessed content validity, while 10 primary healthcare workers participated in face validation. The content validity index (CVI), face validity index (FVI), and modified Kappa were calculated to evaluate clarity, relevance, necessity, and representativeness.

    RESULTS: The initial questionnaire comprised 62 items across two domains (knowledge and readiness). During content validation, one item assessing clarity yielded an item-level CVI (I-CVI) of 0.70 and a modified Kappa of 0.67; other items achieved excellent thresholds (I-CVI ≥ 0.78; modified Kappa ≥ 0.74). Scale-level CVI (S-CVI/Ave) across all criteria was >0.90, indicating excellent overall validity. The universal agreement S-CVI (S-CVI/UA) for relevance was 0.76, slightly below the recommended cut-off. Based on these findings and expert recommendations, one item was removed due to subthreshold performance and redundancy. Face validation demonstrated strong comprehensibility, with item-level FVI (I-FVI), scale-level FVI (S-FVI/Ave), and universal agreement S-FVI (S-FVI/UA) values exceeding recommended thresholds (0.78, 0.90, and 0.83, respectively). The final questionnaire contained 61 items.

    CONCLUSION: This study provides a validated Malay-language questionnaire for assessing knowledge and readiness for mpox management among primary healthcare workers. This tool provides a standardised approach for identifying knowledge gaps and guiding targeted training to strengthen outbreak preparedness.

Related Terms
Filters
Contact Us

Please provide feedback to Administrator (afdal@afpm.org.my)

External Links