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  1. Mohd Salleh S, Mohd Sah SA
    Trop Life Sci Res, 2024 Jul;35(2):51-63.
    PMID: 39234479 DOI: 10.21315/tlsr2024.35.2.3
    This paper highlighted the annual distribution, seasonality and reproduction status of two species of sea turtles in Negeri Sembilan, Peninsular Malaysia between January 2016 and July 2020 (55 months). These data were officially provided by the Department of Fisheries Malaysia (Negeri Sembilan's state), as a part of a conservation effort made by them to protect the endangered species, as the turtle's population residing in Negeri Sembilan is one of the smallest in Malaysia. Current status shows that the hawksbill turtles, Eretmochelys Imbricata population in Negeri Sembilan is in stable decline with an average of <30 nests per year, and an average of <10 nests per year for the green turtle, Chelonia mydas. The overall sum was 122 nests collected for both species from 2016-2020. This paper updated the status of the hawksbill and green turtle after 30 years from the first study recorded by Mortimer et al. in 1993.
  2. Wong SL, Ibrahim S, Abdul Kadir N, Mohd Salleh S
    Appl Health Econ Health Policy, 2019 10;17(5):641-654.
    PMID: 31093937 DOI: 10.1007/s40258-019-00480-9
    BACKGROUND: High medicines prices are a barrier to medicines access, and their impact is greater in developing countries.

    OBJECTIVE: This study assessed the availability, prices and affordability of medicines in public and private sectors in Malaysia to understand the pharmaceutical environment and guide policy recommendations.

    METHODS: This nationwide cross-sectional study adapted the World Health Organization/Health Action International (WHO/HAI) methodology. A total of 87 premises from both public and private sectors participated in this study. Data on 50 medicines were collected to analyze availability, prices and affordability. Medicine prices were compared against the international reference prices (IRPs), and affordability was assessed by daily income level.

    RESULTS: In the public sector, the average availability of generics (74.8%) was higher than that of the originator brand name products (19.4%). However, in the private sector, the availability of originator brands was higher (52.2%) than generics (49.1%). Procurement prices in the public sector were up to 1.5 times the IRPs, but up to 8.4 times in the private sector. The study also observed large price variation across medicines in the private sector. Median retail mark-ups in private hospitals (generics 166.9%; originators 51.0%) were higher than in retail pharmacies (generics 94.7%; originators 22.4%). Generics were generally affordable, but originator brands were unaffordable.

    CONCLUSION: Current policies on generic medicines need to be strengthened to improve the availability and use of generics in the country. High medicine prices and large price variation in the current free market suggest that coherent pricing policies and regulations are needed to safeguard the accessibility and affordability of medicines for the people.

  3. Muhammad Hafiz S, Attah AO, Mohd Salleh S, Rusli MU, Farah Haziqah MT
    Trop Biomed, 2024 Sep 01;41(3):363-369.
    PMID: 39548791 DOI: 10.47665/tb.41.3.017
    Blastocystis a single-celled eukaryotic protist, is known to inhabit the intestines of various hosts, including humans, and has been implicated in a wide spectrum of symptoms, ranging from gastrointestinal issues to skin disorders, thereby establishing its status as an emerging infectious agent. In this study, the prevalence of Blastocystis infection was investigated in insects, including cockroaches, houseflies, and crickets, as well as sea turtles. Additionally, the genotypic characteristics of the isolated Blastocystis strains were examined, and the evolutionary relationships between Blastocystis species found in sea turtles, and animals/humans were determined. Microscopic techniques and molecular methods were utilized in this study. The results showed that four out of 90 insects (4.44%) and one out of 13 sea turtles (7.7%) were infected by Blastocystis. Furthermore, detailed observations revealed the presence of characteristic morphological features, such as vacuolar forms in the cockroach, cricket and sea turtle samples and binary fission from cockroach samples, indicative of Blastocystis' mode of reproduction. While the ST8 of Blastocystis in sea turtles were successfully identified, no subtyping was achieved for the infected insects. This study not only establishes the occurrence of Blastocystis infection in sea turtles but also uncovers its ability to infect insects, suggesting a potential reservoir role for these organisms. Overall, this research emphasizes the significance of comprehending the prevalence, genotypic diversity, and evolutionary relationships of Blastocystis across various hosts. Such insights are instrumental in developing effective control measures and public health interventions to mitigate the associated symptoms and prevent future outbreaks.
  4. Fuentes MMPB, Santos AJB, Abreu-Grobois A, Briseño-Dueñas R, Al-Khayat J, Hamza S, et al.
    Glob Chang Biol, 2024 Jan;30(1):e16991.
    PMID: 37905464 DOI: 10.1111/gcb.16991
    Sea turtles are vulnerable to climate change since their reproductive output is influenced by incubating temperatures, with warmer temperatures causing lower hatching success and increased feminization of embryos. Their ability to cope with projected increases in ambient temperatures will depend on their capacity to adapt to shifts in climatic regimes. Here, we assessed the extent to which phenological shifts could mitigate impacts from increases in ambient temperatures (from 1.5 to 3°C in air temperatures and from 1.4 to 2.3°C in sea surface temperatures by 2100 at our sites) on four species of sea turtles, under a "middle of the road" scenario (SSP2-4.5). Sand temperatures at sea turtle nesting sites are projected to increase from 0.58 to 4.17°C by 2100 and expected shifts in nesting of 26-43 days earlier will not be sufficient to maintain current incubation temperatures at 7 (29%) of our sites, hatching success rates at 10 (42%) of our sites, with current trends in hatchling sex ratio being able to be maintained at half of the sites. We also calculated the phenological shifts that would be required (both backward for an earlier shift in nesting and forward for a later shift) to keep up with present-day incubation temperatures, hatching success rates, and sex ratios. The required shifts backward in nesting for incubation temperatures ranged from -20 to -191 days, whereas the required shifts forward ranged from +54 to +180 days. However, for half of the sites, no matter the shift the median incubation temperature will always be warmer than the 75th percentile of current ranges. Given that phenological shifts will not be able to ameliorate predicted changes in temperature, hatching success and sex ratio at most sites, turtles may need to use other adaptive responses and/or there is the need to enhance sea turtle resilience to climate warming.
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