Ship Sanitation is one of the efforts to overcome several factors of the physical environment that are needed for the main creatures' things that have the damaging effects of physical development, health, and survival. What is done on a transport device that uses a machine or screen that travels nationally or internationally. This type of research uses descriptive methods with a sample of all Ro-Ro vessels at the Merak Ferry Port of 20 ships. Data collection using ship sanitation observation sheets. The results of the study with univariate analysis showed the condition of kitchen sanitation on Ro-Ro vessels which were categorized as not meeting the requirements of 55%, the sanitary conditions of the food raft room category fulfilled the requirements of 85%, the sanitation conditions 80%, the physical conditions of drinking water 100%, the sanitation requirements of the facilities 70% medical, Ro-Ro ship sanitation requirements do not meet the requirements of 53%. Through this research, the authors expect the efforts of related parties to improve the sanitation of Ro-Ro vessels to be safe by increasing sanitation production of clean and healthy Ro-Ro ships.