Introduction: Obesity is a growing public health concern. Poor diet and lifestyle choices are the main contributors to its development. Lifestyle modifications should be aggressively promoted in the community. Recent studies found that worksite obesity prevention and control programs are effective in increasing physical activity and weight reduction among employees. In Malaysia, there is insufficient data on prevalence studies of workplaces. This study investigated the proportion of overweight and obesity among workers in a private university and hope to serve as a baseline for future healthy workplace programs. Methods: This cross-sectional study investigated overweight and obesity and examined its association with occupational stress, sleeping quality, dietary behaviour and physical inactivity among employees in an academic institution. Weight and height were measured, and BMI was calculated and coded as underweight, normal, overweight and obese according to the cut-off points for the Asian population. Results: Out of ninety-five employees, 55 participated in the survey. Among the 55 employees, 4 (7.3%) were underweight, 10 (18.2%) were normal, 25 (45.5%) were overweight, and 16 (29.1%) were obese. The obese and overweight proportion was similar to the national prevalence. Poor sleep quality and high Job stress scores were reported by 40% of the employees, while physical inactivity of more than 6 hours a day was reported by 50%, and the majority (90%) do not consume enough fibre. Among the risk factors investigated, none was associated with obesity. Conclusion: The proportion of overweight and obesity in this community was like the general population. While not significant in this study, the association between obesity and the four risk factors should be further investigated using a larger sample size. Based on the results, community-based health promotion intervention is suggested to reduce obesity among the employees.