A good adhesive is an important factor to consider in orthodontic bonding. Frequent bracket failure prolongs treatment duration and causes inconvenience to patient and operator. This study aimed to compare the effectiveness of two bonding agents, i.e. the self etching primer (SEP) and the conventional etch and bond (CEB) by monitoring the incidence, time and
sites of bracket failure. Eighty orthodontic patients were recruited after informed consent were obtained. A randomized split mouth technique was used where one side was bonded with CEB agent, while the other side was bonded using SEP agent. All bonded brackets were examined every 4 weeks for 20 months. Incidence of bracket failure was noted and
comparison between the two groups was done using paired t-test. Time and frequent site of failure were also assessed. Out of 1314 brackets, only 33 brackets were debonded with 16 from SEP group and 17 from CEB group which statistically insignificant (p>0.05). Almost 55% of bracket failure occurred during the first 3 months after bonding for both adhesives
with lower premolars was most the frequent site to fail in both adhesives (39.4%). Bracket bonded on the left side failed significantly compared to the right side. In conclusion, both self-etching primer and conventional acid-etch bonding agents are equally effective in retaining brackets clinically. Bracket failure mostly occurres during the first 3 months after bonding. The premolar brackets and the left side are mostly debonded when compared to other sites.
Metallo-β-lactamase (MBL) is an enzyme produced by clinically important bacteria that can inactivate many commonly used antibiotics, making them a significant concern in treating bacterial infections and the risk of having high antibiotic resistance issues among the community. This review presents a bibliometric and patent analysis of MBL worldwide research trend based on the Scopus and World Intellectual Property Organization databases in 2013-2022. Based on the keywords related to MBL in the article title, abstract, and keywords, 592 research articles were retrieved for further analysis using various tools such as Microsoft Excel to determine the frequency analysis, VOSviewer for bibliometric networks visualization, and Harzing's Publish or Perish for citation metrics analysis. Standard bibliometric parameters were analysed to evaluate the field's research trend, such as the growth of publications, topographical distribution, top subject area, most relevant journal, top cited documents, most relevant authors, and keyword trend analysis. Within 10 years, MBL discovery has shown a steady and continuous growth of interest among the community of researchers. United States of America, China, and the United Kingdom are the top 3 countries contribute high productivity to the field. The patent analysis also shows several impactful filed patents, indicating the significance of development research on the structural and functional relationship of MBL for an effective structure-based drug design (SBDD). Developing new MBL inhibitors using SBDD could help address the research gap and provide new successful therapeutic options for treating MBL-producing bacterial infections.
This review (with 99 refs.) summarizes the progress that has been made in colorimetric (i.e. spectrophotometric) determination of organophosphate pesticides (OPPs) using gold and silver nanoparticles (NPs). Following an introduction into the field, a first large section covers the types and functions of organophosphate pesticides. Methods for colorimetric (spectrophotometric) measurements including RGB techniques are discussed next. A further section covers the characteristic features of gold and silver-based NPs. Syntheses and modifications of metal NPs are covered in section 5. This is followed by overviews on enzyme inhibition-based assays, aptamer-based assays and chemical (non-enzymatic) assays, and a discussion of specific features of colorimetric assays. Several Tables are presented that give an overview on the wealth of methods and materials. A concluding section addresses current challenges and discusses potential future trends and opportunities. Graphical abstractSchematic representation of organophosphate pesticide determinations based on aggregation of nanoparticles (particular silver or gold nanoparticles). This leads to a color change which can be determined visually and monitored by a red shift in the absorption spectrum.