Displaying all 2 publications

  1. Tan Hc, Tan Bc, Norzulaani Khalid, Wong Sm
    Sains Malaysiana, 2016;45:795-802.
    Boesenbergia rotunda is a medicinal ginger that has been found to contain several bioactive compounds such as
    boesenbergin A, panduratin A, cardamonin, pinostrobin and pinocembrin. These compounds are useful in treating various
    ailments, such as oral diseases, inflammation and have also been used as an aphrodisiac. In this study, an efficient
    protocol for developing and isolating protoplast cultures for B. rotunda has been established. Rhizome buds of B. rotunda
    were used as explants to initiate callus growth and the established cell suspension cultures were used to optimize their
    growth conditions. Our results indicated that embryogenic suspension cultures in liquid Murashige and Skoog (MS)
    medium supplemented with 3% (w/v) sucrose produced the highest growth rate (µ = 0.1125), whereas no promotive effect
    was seen in the presence of 2,4-dichlorophenoxyacetic acid and those that underwent sonication treatment. Amount of
    protoplasts isolated ranging from 1-5 × 105
    protoplast per mL were isolated using 0.25% (w/v) macerozyme and 1%
    (w/v) cellulase for 24 h under continuous agitation (50 rpm) in dark condition. Of the isolated protoplasts, 54.93% were
    viable according to fluorescein diacetate staining test. Micro-colonies were recovered in liquid MS medium containing
    9 g/L mannitol, 2 mg/L 1-naphthaleneacetic acid and 0.5 mg/L benzylaminopurine (BAP) for 4 weeks and subsequently
    transferred to solid MS medium supplemented with 0.5 mg/L BAP for callus initiation. The protoplast system established
    in this study would be useful for genetic manipulation and modern breeding program of B. rotund
  2. Nadiya Akmal Baharum, Rofina Yasmin Othman, Boon Chin Tan, Kamilatulhusna Zaidi, Norzulaani Khalid, Nadiya Akmal Baharum, et al.
    Sains Malaysiana, 2018;47:2291-2300.
    PR-10 is a member of pathogenesis-related (PR) genes elicited by the plant’s defense mechanism during pathogen attack.
    Elevated expression of PR-10 upon different pathogen invasions has been observed in many plant species suggesting
    its role as an anti-bacterial, anti-viral and anti-fungal gene. However, the effect of PR-10 in mitigating the infection of
    Fusarium oxysporum f. sp. cubense (Foc), the causal agent of Fusarium wilt in banana has not been reported. In this
    study, the coding sequences of PR-10 gene isolated from Foc resistant Musa acuminata ssp. malaccensis (MaPR-10)
    were integrated into a local Foc susceptible commercial banana cultivar, Berangan via co-cultivation of embryogenic
    cell suspension and Agrobacterium tumefaciens. Out of 17 putative transgenic lines established, 11 of them positively
    harbored MaPR-10. Among these, Line-19 plantlets showed the most rapid in-vitro propagation and successfully overexpressed the transgene. Following a nursery challenge experiment with a virulent Foc race 4 (CI HIR) isolate, about 30%
    of Line-19 plants showed a one-week delay in disease progression when compared to the untransformed controls. From
    the final evaluation performed in the 5th week-post-inoculation, the leaf symptoms index (LSI) and rhizome discoloration
    index (RDI) of Line-19 was 3.4 and 6.1, respectively, indicating the disease had progressed. The findings of this study
    enrich the current existing knowledge on the roles of PR-10 in combating fungal disease in plants.
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