The mistletoe fig (Ficus deltoidea) is frequently found in several areas of the world, and primarily functions as houseplant or an ornamental shrub. The plant is discovered indigenous generally in Asia tropical region for example Indonesia, Philippines, Malaysia, and Thailand. Scientific studies on the effect of plant growth regulators on cells production from this plant are vital as optimization of cells production may result in effective production of secondary products characterization and output. The growth of cell suspension cultures from this plant shows sigmoidal property. In this work, we model the effect of the plant growth regulator 2,4-dichlorophenoxyacetic acid (2,4-D) on the growth kinetics of the cells from this plant according to the modified Gompertz model. The coefficient of determination showed good agreement between experimental and predicted data with values ranging from 0.97-0.98. The results showed that 2,4-D at 2 mg/L was optimal for achieving the highest cells growth rate. It is anticipated that the growth parameter constants extracted from the modelling exercise will be helpful in the future for additional secondary modelling on the effect of media conditions as well as other factors on cells growth.
Ficus deltoidea (or commonly known as mistletoe fig) in various parts of the world mainly
serves as an ornamental shrub or houseplant and found native mainly in Asia tropical region,
for example, Malaysia, Indonesia, Philippines and Thailand. Studies on the effect of plant
growth regulators on cells production from this plant is important as optimization of cells
production can lead to efficient production of secondary products characterization and
production. An important aspect of the sigmoidal cells growth curve is the growth parameters.
In this work, we model the effect of picloram (4-amino-3,5,6-trichloropicolinic acid) on the
growth kinetics of the cell suspension cultures of Ficus deltoidea according to the modified
Gompertz model. The adjusted coefficient of determination showed good agreement between
experimental and predicted data with values ranging from 0.97-0.99. Parameters obtained from
the fitting exercise were maximum cells growth rate (μm), lag time (l) and maximal cells
production (Ymax). The results showed that picloram at concentrations of 3 mg/L and above
were optimal for giving the highest cells growth rate measured as PCV (packed cell volume).
The parameter growth rate obtained from the modelling exercise will be helpful for additional
secondary modelling implicating the consequence of media conditions as well as other factors
on the effect of picloram on the growth rate of cell suspension from this plant.
Forty three (n=43) genomic DNA of Escherichia coli (11 isolates from eggs and 32 isolates from imported beef meats) were characterized by shiga toxin 1 (stx1), enterobacterial repetitive intergenic consensus-PCR (ERIC-PCR) and random amplified polymorphic DNA-PCR (RAPD-PCR) analyses. In the shiga toxin 1 (stx1) gene detection with primer stx 1F (5’-TTCTTCGGTATCCTATTCCC-3’) and stx 1R (5’- CTGTCACAGTAACAACCGT-3’), 9 E. coli of beef meats isolates were positive toward sxt1 gene. The results of the ERIC-PCR and RAPD-PCR were analyzed using GelCompar II software. ERIC-PCR with primer ERIC1 (5’-CACTTAGGGGTCCTCGAATGTA -3’) and ERIC2 (5’-AAGTAAGTGACTGGGGTGAGCG-3’) discriminated the E. coli into 6 clusters and 10 single isolates at 80% similarity. RAPD-PCR with primer Gen8 and Gen9, produced 10 clusters and 15 single isolates and 12 clusters and 14 single isolates of 80%, respectively. These results demonstrated that both ERIC-PCR and RAPD-PCR are useful and suitable tools for molecular typing of those isolates examined.