Kaempferia galangal rhizome is one of the traditional medicinal plant species in
Zingiberaceae family and well known as “cekur” in Borneo. Rapid emergence of
multidrug resistance of V. cholerae biofilm has increased the failure of antibiotic
treatment in recent years. This research was designed to demonstrate the
antimicrobial activity of K. galangal rhizome extract against twenty six (n=26) V. cholera
biofilm which were isolated from outbreak in Limbang, Sarawak in 2016. K. galangal
rhizome was analyzed by methanol extraction and tested against biofilm of V. cholerae.
The susceptibility of V. cholerae towards K. galangal rhizome was evaluated using disk
diffusion method which showed a maximum zone of inhibition of 12.0 mm at 1000
mg/mL concentration. As a result, the MBEC50 of V. cholerae was between 125 mg/mL
to 250 mg/mL while more than 90% biofilm eradication (MBEC90) was achieved by 500
mg/mL extract concentration. Extract-treated cell showed change in the morphology
of V. cholerae by destruction of cell wall. K. galangal rhizome extract acts as a potent
antibiofilm agent with dual actions by preventing and eradicating the biofilm of V.
cholerae.