METHODOLOGY: TruNatomy (Dentsply Sirona, USA), 2Shape (Micro-Mega, France), and One Curve (Micro-Mega, France) were the three different rotary files that were employed. A total of 45 excised human permanent first mandibular molars were divided into Groups A (TruNatomy), B (2Shape), and C (One Curve) at random. To measure the residual dentin thickness at 3 mm, 5 mm, and 7 mm from the radiographic apex, the mesial root of the tooth was removed from the tooth, and a mesio-buccal canal was taken. Samples were mounted in clear acrylic resin and were subjected to a pre-instrumentation CBCT scan. The mesio-buccal canal was cleaned and shaped while maintaining the final mesio-buccal canal preparation of Group A - 26/0.04, Group B - 25/0.04, and Group C - 25/0.04. The samples were extensively irrigated with 3% sodium hypochlorite and 17% EDTA, and a post-instrumentation scan was performed on them. Statistics were used to determine the values from CBCT scans that were recorded for pre- and post-instrumentations.
RESULTS: The results showed that Group A had the greatest drop in dentin thickness, followed by Group B and Group C. The change in dentin thickness was greatest at 3 mm and 7 mm.
CONCLUSION: In contrast to TruNatomy and 2Shape rotary file systems, One Curve has the advantage of maintaining a tooth's thickness at 3 and 7 millimeters from the radiological apex. Since the TruNatomy file system removes more dentin than the other two combined, it should be used cautiously. Choosing the right instrument is crucial for cleaning and shaping during root canal preparation.