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  1. Safri Zainal Abidin, Raizulnasuha Abdul Rashid, Muhammad Afiq Khairil Anuar, Wan Nordiana A. Abd Rahman
    MyJurnal
    The purpose of this study is to determine the technetium-99m pertechnetate (99mTcO4) intercellular uptake by different types of cell lines. HeLa, human fetal
    osteoblast (hFOB), glial and glioma cell lines grown in 6-wells culture plates were incubated with 99mTcO4 of activity of 200, 400, 600. 800 and 1000
    pCi for 30 minutes at 3T°C and 5% ( '< - humidified atmosphere. After incubation, the cells were washed 3 times with phosphate buffer saline to remove the
    extracellular traces of 99mTcO4. Measurements of the intercellular Q9mTcOjt radioactivity were performed using single head gamma
    camera and the percentage uptake of the yUwTcGpinto the cells was calculated. The intercellular uptake
    0fyUmTcO_( was found to be inversely correlate to the radioactivity HeLa cell shows the highest uptake
    followed by hFOB, glial and glioma cell lines. Comparison of uptake between normal and cancer cells
    present indistinguishable results. The findings of this study suggest that the intercellular uptake of
    yymTcOjt is highly dependent on the type of cells despite no significant different of uptake was found
    between normal and cancer cell lines. The level of radioactivity is also an important determinant
    factor that influence the uptake ofyUmTcG) into the cells. This study will be the first precedent toward
    understanding the cellular characteristic and pharmacokinetic of non-invasive imaging tracer for
    future molecular imaging and therapy.
  2. Muhammad Afiq Khairil Anuar, Siti Zanariah Ab Aziz, Raizulnasuha Abdul Rashid, Safri Zainal Abidin, Norhayati Dollah, Wan Nordiana A. Abd Rahman
    MyJurnal
    Radiotherapy has become the most important modality in treating cancer with approximately 50% of
    cancer patient undergo the treatment. However, more improvement to the radiotherapy treatment
    efficacy is required to deprive cancer. Assessment of tumor progress during treatment is important, to
    accommodate the changes that occur during the fractionation course. The objective of this study is to
    assess tumor cell damage after external beam radiotherapy by using technetium-99m
    pertechnetate (99mTcOf) as a tracer. In this study, HeLa cells were irradiated with 6 MVphoton beam
    with different radiation dose ranging from 0.5 Gy to 10 Gy. The irradiated cells were recultured in 6-
    well plates and incubated for 10 days. After that, 2 mCi of 99mTcOf were prescribed to each cell
    colonies. The viable cells were separated from the rest, and measured for 99mTcOf uptake using singlehead
    gamma camera with LEHR collimation. As results, the cells survival, fractions clearly indicate
    diminishing effect, to the cells at, higher dose of irradiation. Good correlation were observed between
    mmTcGi uptake and survival, fraction for cells irradiated at, lower dose and less significant, correlation
    were indicated at higher dose. In conclusion, there is potential for the efficacy of external beam
    radiotherapy in treating cancer to be assessed by using radioisotope as a non-invasive tracer. In this
    case, technetium-99m, pertechnetate (99mTcOjt) could be attached to the specific antibody so that, better
    correlation, between, the cells uptake and possible cell damages could be observed.
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