This data article describes the growth performance, glucose concentration in blood and testosterone level in plasma of juvenile Asian seabass (Lates calcarifer) after being fed with exogenous melatonin at different concentrations (0, 50 and 100 mg kg-1). To collect the data, 160 juveniles (60 days old) with an initial mean weight of 20.54 ± 7.16 g and mean length 11.14 ± 0.05 cm were reared in 1800 L rectangular fibreglass aerated tanks of a recirculating aquaculture system (n = 40 fish/ tank/ treatment) for 90 days. Four different treatments were tested: basal diet without any hormone (Control), basal diet with ethanol solution (Control + Ethanol; vehicle control), basal diet sprayed with 50 mg/kg feed of melatonin (Melatonin (50 mg/kg of diets)) and basal diet sprayed with 100 mg/kg feed of melatonin (Melatonin 100 mg/kg of diets). Initial and final body weight and body length of fish was measured and recorded to calculate the data of weight gain (WG). At the end of the feeding trial, the juveniles blood glucose and testosterone level were analyzed. Normality test, One-way ANOVA analysis followed by Tukey post-hoc test were then performed on the data obtained from the calculation of WG, survival rate, blood glucose and testosterone level. The data presented in this article will helps farmers and scientist to optimize the usage of melatonin administration in Asian seabass juveniles.