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  1. Fulltext Development and validation of a gamified sexual and reproductive health education module for adolescent boys in Malaysia
    Saini N, Saimon R, Safii R, Minoi JL
    Malays Fam Physician, 2025;20:1.
    PMID: 39949648 DOI: 10.51866/oa.645
    INTRODUCTION: About one-third of Malaysian adolescents engage in sexual activities before the age of 14 years, putting them at risk for unplanned pregnancies and sexually transmitted diseases. However, implementing sex education in Malaysia remains controversial and challenging. Therefore, this study aimed to develop and validate a newly gamified sexual and reproductive health (SRH) education module (ReReki) tailored for Malaysian adolescent boys.

    METHODS: The ReReki module was developed using the analysis, design, development, implementation and evaluation model, based on the theory of planned behaviour. Content validation involved six adolescent health experts, using Russell's model validity approach and a content validity questionnaire. Games were then designed to complement the Sexual and Reproductive Health (SRH) materials. The gamified ReReki module was pre-tested with 20 adolescent boys from a selected school, and the language was refined by a linguistic expert.

    RESULTS: The module included five main topics, 29 subtopics and six games. The overall content validity score based on the survey method was 81.8%, while that based on Russell's model was 79.3%, indicating a high level of validity for the ReReki module. One new topic and two subtopics were added, while two subtopics scoring below 70% were flagged for further review. The adolescent boys expressed their acceptance of the gamified SRH content.

    CONCLUSION: All five main topics, along with the 29 subtopics in the ReReki module, are suitable and ready for use by adolescent boys in the Malaysian context.

  2. The survival of influenza A(H1N1)pdm09 virus on 4 household surfaces
    Oxford J, Berezin EN, Courvalin P, Dwyer DE, Exner M, Jana LA, et al.
    Am J Infect Control, 2014 Apr;42(4):423-5.
    PMID: 24679569 DOI: 10.1016/j.ajic.2013.10.016
    We investigated the survival of a pandemic strain of influenza A H1N1 on a variety of common household surfaces where multiple samples were taken from 4 types of common household fomite at 7 time points. Results showed that influenza A H1N1sw virus particles remained infectious for 48 hours on a wooden surface, for 24 hours on stainless steel and plastic surfaces, and for 8 hours on a cloth surface, although virus recovery from the cloth may have been suboptimal. Our results suggest that pandemic influenza A H1N1 can survive on common household fomites for extended periods of time, and that good hand hygiene and regular disinfection of commonly touched surfaces should be practiced during the influenza season to help reduce transmission.
  3. Fulltext Integrated genomic view of SARS-CoV-2 in India
    Kumar P, Pandey R, Sharma P, Dhar MS, A V, Uppili B, et al.
    Wellcome Open Res, 2020;5:184.
    PMID: 32995557 DOI: 10.12688/wellcomeopenres.16119.1
    Background: India first detected SARS-CoV-2, causal agent of COVID-19 in late January 2020, imported from Wuhan, China. From March 2020 onwards, the importation of cases from countries in the rest of the world followed by seeding of local transmission triggered further outbreaks in India. Methods: We used ARTIC protocol-based tiling amplicon sequencing of SARS-CoV-2 (n=104) from different states of India using a combination of MinION and MinIT sequencing from Oxford Nanopore Technology to understand how introduction and local transmission occurred. Results: The analyses revealed multiple introductions of SARS-CoV-2 genomes, including the A2a cluster from Europe and the USA, A3 cluster from Middle East and A4 cluster (haplotype redefined) from Southeast Asia (Indonesia, Thailand and Malaysia) and Central Asia (Kyrgyzstan). The local transmission and persistence of genomes A4, A2a and A3 was also observed in the studied locations. The most prevalent genomes with patterns of variance (confined in a cluster) remain unclassified, and are here proposed as A4-clade based on its divergence within the A cluster. Conclusions: The viral haplotypes may link their persistence to geo-climatic conditions and host response. Multipronged strategies including molecular surveillance based on real-time viral genomic data is of paramount importance for a timely management of the pandemic.
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