Mosquito identification and classification are the most important steps in a surveillance program of mosquito-borne diseases. With conventional approach of data collection, the process of sorting and classification are laborious and time-consuming. The advancement of computer vision with transfer learning provides excellent alternative to the challenge. Transfer learning is a type of machine learning that is viable and durable in image classification with limited training images. This protocol aims to develop step-by-step procedure in developing a classification system with transfer learning algorithm for mosquito, we demonstrate the protocol to classify two species of Aedes mosquito - Aedes aegypti L. and Aedes albopitus L, but user can adopt the protocol for higher number of species classification. We demonstrated the way of start from the scratch, fine-tuning two pre-trained model performance by using different combination of hyperparameters - batch size and learning rate, and explain the terminology in the Appendix. This protocol target on the domain expert such as entomologist and public health practices to develop their own model to solve the task of mosquito/insect classification.
Radiation shielding incorporates material between the radioactive source and environment to decrease exposure to hazardous radiation. It remains to be seen whether the addition of nanoparticles effectively increases the protection of tellurite glass system from further degradation under irradiation conditions. This study revealed the gamma radiation effects on tellurite glass. The tellurite glass samples were irradiated with 50 kGy and 100 kGy gamma ray, and subsequently analysed using X-ray diffractometer (XRD), atomic force microscopy (AFM), and ultraviolet-visible spectroscopy (UV-Vis). Gamma radiation increased the creation of non-bridging oxygen (NBO) and caused colour change on TZNETi and TZNETiAl glasses. Consequently, the addition of aluminium oxides (Al2O3) was found to lower the density of glass systems. The glass samples surface roughness increased, while the optical transmission spectra decreased after 50 kGy of gamma ray irradiation. Nevertheless, the glass system maintained its transparency even after irradiation. The mass attenuation coefficient (MAC) values represented the shielding effectiveness demonstrated by the investigated glass with the addition of Al2O3. The physical, structural, optical, and radiation shielding properties showed that 69.1TeO2-20ZnO-9Na2O-1Er2O3-0.3TiO2-0.6Al2O3 (TZNETiAl) sample exhibited strong shielding properties amongst the fabricated tellurite samples.