METHODS: A total of 58 bacterial isolates, previously recovered from various clinical specimens, were tested. Genotypic identification was performed using a multiplex PCR assay targeting carbapenemases, including blaKPC, blaNDM, and blaOXA-48. The rapid assay was conducted using the NG-CARBA-5 test kit to detect blaNDM, blaIMP, blaVIM, blaOXA-48, and blaKPC.

RESULTS: Out of the 58 isolates tested, 36 were identified as carbapenemase producers with the rapid assay, whereas 39 were detected by PCR. All 36 carbapenemase-positive isolates detected by the rapid assay harboured the blaNDM gene, while multiplex PCR detected 19 isolates with multigenes (18 blaNDM+blaKPC and 1 blaNDM+blaOXA) and 20 isolates with single genes (18 blaNDM, 1 blaOXA, and 1 blaKPC). Discrepancies were observed among the detection methods, with four isolates reported as false negatives (FNs). This indicates a possible risk of missing colonised or infected patients, which is important in settings such as intensive care units or outbreaks.