Data in this article are supplementary to the corresponding research article [1]. Morphological features of homogeneous and graded nanofibrous electrospun gelatin scaffolds were observed using scanning electron microscopy. Microstructural properties including fiber diameter and pore size were determined via image analysis, using ImageJ. Uniaxial tensile and fracture tests were performed on both homogeneous and graded scaffolds using a universal testing machine. Stress-strain curves of all scaffolds are presented. Computing software, MATLAB, was used to design fibrous networks with thickness-dependent density and alignment gradients (DAG). Finite element analysis software, Abaqus, was used to determine the effect of the number of layers on the fracture properties of DAG multilayer scaffolds.