Burkholderia pseudomallei is the causative agent of melioidosis, an
infectious disease endemic in Southeast Asia and northern Australia. Cases have been
reported in Pahang, Johor Bahru and Kedah. The disease is difficult to combat as B.
pseudomallei has shown resistance to various antibiotics and much is still not understood
about its pathogenicity. It is suggested that investigating the bacterium hypothetical
proteins may provide potential new targets for the development of antimicrobials. The
gene of interest in this study, BPSL2774, encoding BPSL2774 hypothetical protein, is a
target gene that was predicted as essential using transposon-directed insertion site
sequencing technique (TraDIS). We aimed to express and purify soluble GST-tagged
BPSL2774 protein at sufficient concentration for future functional assays. (Copied from article).