Displaying all 15 publications

Abstract:
Sort:
  1. Suardi N, Sodipo BK, Mustafa MZ, Ali Z
    J. Photochem. Photobiol. B, Biol., 2016 Jul 28;162:703-706.
    PMID: 27508880 DOI: 10.1016/j.jphotobiol.2016.07.041
    In this work we present influence of visible laser light on ATP level and viability of anaemic red blood cell (RBC). The visible laser lights used in this work are 460nm and 532nm. The responses of ATP level in anaemic and normal RBC before and after laser irradiation at different exposure time (30, 40, 50 and 60s) were observed. Three aliquots were prepared from the ethylenediaminetetraacetic acid (EDTA) blood sample. One served as a control (untreated) and another two were irradiated with 460nm and 560nm lasers. Packed RBC was prepared to study ATP level in the RBC using CellTiter-GloLuminescent cell Viability Assay kit. The assay generates a glow type signal produced by luciferase reaction, which is proportional to the amount of ATP present in RBCs. Paired t-test were done to analyse ATP level before and after laser irradiation. The results revealed laser irradiation improve level of ATP in anaemic RBC. Effect of laser light on anaemic RBCs were significant over different exposure time for both 460nm (p=0.000) and 532nm (p=0.003). The result of ATP level is further used as marker for RBC viability. The influence of ATP level and viability were studied. Optical densities obtained from the data were used to determine cell viability of the samples. Results showed that laser irradiation increased viability of anaemic RBC compared to normal RBC.
  2. Suardi N, Germanam SJ, Rahim NAYM
    Lasers Med Sci, 2023 Apr 14;38(1):99.
    PMID: 37059895 DOI: 10.1007/s10103-023-03766-6
    Although positive photobiomodulation response on wound healing, tissue repair, and therapeutic treatment has been widely reported, additional works are still needed to understand its effects on human blood. This research carried out acoustic measurements using A-scan (GAMPT) ultrasonic techniques to elucidate the photobiomodulation effects on in vitro human blood samples as therapeutic treatment measures. The human blood samples were irradiated using a 532-nm laser with different output laser powers (60 and 80 mW) at various exposure times. The ultrasonic velocity measured in the human blood samples after laser irradiation showed significant changes, most of which were within the acceptance limit for soft tissues (1570 [Formula: see text] 30 m/s). Abnormal cells (echinocyte and crenation) were observed due to excessive exposure during laser treatment.
  3. Musawi MS, Jafar MS, Al-Gailani BT, Ahmed NM, Suhaimi FM, Suardi N
    Photomed Laser Surg, 2016 May;34(5):211-4.
    PMID: 26966989 DOI: 10.1089/pho.2015.4043
    OBJECTIVE: This study was conducted to investigate the effects of low-level laser (LLL) doses on human red blood cell volume. The effects of exposure to a diode pump solid state (DPSS) (λ = 405 nm) laser were observed.

    BACKGROUND DATA: The response of human blood to LLL irradiation gives important information about the mechanism of interaction of laser light with living organisms. Materials and methods Blood samples were collected into ethylenediaminetetraacetic acid (EDTA)-containing tubes, and each sample was divided into two equal aliquots, one to serve as control and the other for irradiation. The aliquot was subjected to laser irradiation for 20, 30, 40, or 50 min at a fixed power density of 0.03 W/cm(2). Mean cell volume (MCV) and red blood cell (RBC) counts were measured immediately after irradiation using a computerized hemtoanalyzer.

    RESULTS: Significant decrease in RBC volume (p 

  4. Ahmad MS, Suardi N, Shukri A, Nik Ab Razak NNA, Oglat AA, Makhamrah O, et al.
    Eur J Radiol Open, 2020;7:100257.
    PMID: 32944594 DOI: 10.1016/j.ejro.2020.100257
    Introduction: Hepatocellular carcinoma (HCC) is one of the most common cancer in the world, and the effectiveness of its treatment lies in its detection in its early stages. The aim of this study is to mimic HCC dynamically through a liver phantom and apply it in multimodality medical imaging techniques including magnetic resonance imaging (MRI), computed tomography (CT), and ultrasound.

    Methods and materials: The phantom is fabricated with two main parts, liver parenchyma and HCC inserts. The liver parenchyma was fabricated by adding 2.5 wt% of agarose powder combined with 2.6 wt% of wax powder while the basic material for the HCC samples was made from polyurethane solution combined with 5 wt% glycerol. Three HCC samples were inserted into the parenchyma by using three cylinders implanted inside the liver parenchyma. An automatic injector is attached to the input side of the cylinders and a suction device connected to the output side of the cylinders. After the phantom was prepared, the contrast materials were injected into the phantom and imaged using MRI, CT, and ultrasound.

    Results: Both HCC samples and liver parenchyma were clearly distinguished using the three imaging modalities: MRI, CT, and ultrasound. Doppler ultrasound was also applied through the HCC samples and the flow pattern was observed through the samples.

    Conclusion: A multimodal dynamic liver phantom, with HCC tumor models have been fabricated. This phantom helps to improve and develop different methods for detecting HCC in its early stages.

  5. Suardi N, Khaniabadi PM, Taggo A, Zulbaharin SFM, Azman DKM, Gemanam SJ
    Lasers Med Sci, 2021 Jul 31.
    PMID: 34331605 DOI: 10.1007/s10103-021-03384-0
    Breast cancer is responsible for one of the top leading causes of cancer deaths among women. Radiotherapy (RT) uses high energy radiation to kill cancer cells, but this method has been reportedly linked to risks of toxicity. Post-therapeutic relapse from RT believed to be caused by its toxicity is one of the challenges encountered during tumour therapy. Therefore, further attention should be devoted to developing novel anti-tumour therapeutic approaches. The role of low-level laser therapy (LLLT) in breast cancer management is to alleviate the side effects arising from RT, instead of acting against the tumour cells directly. This study investigated the effects of low-level laser (532 nm), as well as single and fractionated irradiation, on breast cancer MCF 7 cell line. Additionally, this study assessed the most effective laser parameter for fractionated irradiation. The MCF 7 cells were irradiated with green laser power at 1.5, 45.0, and 100.0 mW with a spot size diameter of 0.7 mm for 1, 5, 10, and 15 min. The irradiation was carried out in single, double, and triple fractionation separated by 5- and 10-min intervals in between the fractional regimes. The laser output of 100 mW showed a promising potential in killing cells with single fractionation. However, as the irradiation was fractionated into two, power of 1.5 mW appeared to be more effective in cell death, which contributed to the lowest percentage cells viable of 31.4% recorded in the study. It was proven that fractionated regime was more successful in tumour cell death.
  6. Dakok KK, Matjafri MZ, Suardi N, Oglat AA, Nabasu SE
    J Ultrason, 2021 Aug 16;21(86):e219-e224.
    PMID: 34540276 DOI: 10.15557/JoU.2021.0035
    Aim of the study: At present, there are few scatter particles used in preparing blood-mimicking fluids, such as nylon, sephadex, polystyrene microsphere, and poly(4-methystyrene). In this study, we present cholesterol as a new scatter particle for blood-mimicking fluid preparation. Materials and methods: The procedure for the preparation of the proposed blood-mimicking fluid involved the use of propylene glycol, D(+)-Glucose and distilled water to form a ternary mixture fluid, with cholesterol used as scatter particles. Polyethylene glycol was first used as part of the mixture fluid but the acoustic and physical properties were not suitable, leading to its replacement with D(+)-Glucose, which is soluble in water and has a higher density. A common carotid artery wall-less phantom was also produced to assess the flow properties. Results: The prepared blood-mimicking fluid with new scatter particles has a density of 1.067 g/cm3, viscosity of 4.1 mPa.s, speed of sound 1600 m/s, and attenuation of 0.192 dB/cm at 5 MHz frequency. Peak systolic velocity, end diastolic velocity and mean velocity measurements were gotten to be 40.2 ± 2.4 cm/s, 9.9 ± 1.4 cm/s, and 24.0 ± 1.8 cm/s, respectively. Conclusion: Based on the results obtained, the blood-mimicking fluid was found suitable for ultrasound applications in carotid artery wall-less phantoms because of its good acoustic and physical properties.
  7. Al Musawi MS, Jaafar MS, Al-Gailani B, Ahmed NM, Suhaimi FM, Suardi N
    Lasers Med Sci, 2017 Feb;32(2):405-411.
    PMID: 28044209 DOI: 10.1007/s10103-016-2134-1
    Low-level laser irradiation (LLLI) has various effects on cultured human lymphocytes in vitro, but little is known about such effects in whole blood. This study investigated whether LLLI affected lymphocyte count in human whole blood in vitro. A total number of 130 blood samples were collected from apparently healthy adult patients through venipuncture into tubes containing EDTA. Each sample was divided into two equal aliquots to be used as a non-irradiated control sample and an irradiated sample. The irradiated aliquot was subjected to laser wavelengths of 405, 589, and 780 nm with different fluences of 36, 54, 72, and 90 J/cm(2), at a fixed irradiance of 30 mW/cm(2). A paired student t test was used to compare between non-irradiated and irradiated samples. The lymphocyte counts were measured using a computerized hematology analyzer and showed a significant (P 
  8. Oglat AA, Matjafri MZ, Suardi N, Oqlat MA, Abdelrahman MA, Oqlat AA
    J Med Ultrasound, 2018 03 28;26(1):3-13.
    PMID: 30065507 DOI: 10.4103/JMU.JMU_11_17
    Medical Doppler ultrasound is usually utilized in the clinical adjusting to evaluate and estimate blood flow in both the major (large) and the minor (tiny) vessels of the body. The normal and abnormal sign waveforms can be shown by spectral Doppler technique. The sign waveform is individual to each vessel. Thus, it is significant for the operator and the clinicians to understand the normal and abnormal diagnostic in a spectral Doppler show. The aim of this review is to explain the physical principles behind the medical Doppler ultrasound, also, to use some of the mathematical formulas utilized in the medical Doppler ultrasound examination. Furthermore, we discussed the color and spectral flow model of Doppler ultrasound. Finally, we explained spectral Doppler sign waveforms to show both the normal and abnormal signs waveforms that are individual to the common carotid artery, because these signs are important for both the radiologist and sonographer to perceive both the normal and abnormal in a spectral Doppler show.
  9. Ahmad MS, Suardi N, Shukri A, Mohammad H, Oglat AA, Alarab A, et al.
    J Med Ultrasound, 2020 01 28;28(1):7-16.
    PMID: 32368444 DOI: 10.4103/JMU.JMU_4_19
    Liver phantoms have been developed as an alternative to human tissue and have been used for different purposes. In this article, the items used for liver phantoms fabrication are mentioned same as in the previous literature reviews. Summary and characteristics of these materials are presented. The main factors that need to be available in the materials used for fabrication in computed tomography, ultrasound, magnetic resonance imaging, and nuclear medicine were analyzed. Finally, the discussion focuses on some purposes and aims of the liver phantom fabrication for use in several areas such as training, diagnoses of different diseases, and treatment planning for therapeutic strategies - for example, in selective internal radiation therapy, stereotactic body radiation therapy, laser-induced thermotherapy, radiofrequency ablation, and microwave coagulation therapy. It was found that different liver substitutes can be developed to fulfill the different requirements.
  10. Dakok KK, Matjafri MZ, Suardi N, Oglat AA, Nabasu SE
    J Med Ultrasound, 2021 05 14;29(3):157-166.
    PMID: 34729323 DOI: 10.4103/JMU.JMU_164_20
    Ultrasound imaging systems need tissue-mimicking phantoms with a good range of acoustic properties. Many studies on carotid artery phantoms have been carried out using ultrasound; hence this study presents a review of the different forms of carotid artery phantoms used to examine blood hemodynamics by Doppler ultrasound (DU) methods and explains the ingredients that constitute every phantom with their advantages and disadvantages. Different research databases were consulted to access relevant information on carotid artery phantoms used for DU measurements after which the information were presented systematically spanning from walled phantoms to wall-less phantoms. This review points out the fact that carotid artery phantoms are made up of tissue mimicking materials, vessel mimicking materials, and blood mimicking fluid whose properties matched those of real human tissues and vessels. These materials are a combination of substances such as water, gelatin, glycerol, scatterers, and other powders in their right proportions.
  11. Oglat AA, Suardi N, Matjafri MZ, Oqlat MA, Abdelrahman MA, Oqlat AA
    J Med Ultrasound, 2018;26(2):68-76.
    PMID: 30065522 DOI: 10.4103/JMU.JMU_1_17
    Doppler ultrasound imaging system description and calibration need blood-mimicking fluids (BMFs) for the test target of medical ultrasound diagnostic tools, with known interior features and acoustic and physical properties of this fluid (BMF). Physical and acoustical properties determined in the International Electrotechnical Commission (IEC) standard are specified as constant values, the materials used in the BMF preparation should have values similar to the IEC standard values. However, BMF is ready-made commercially from a field of medical usage, which may not be appropriate in the layout of ultrasound system or for an estimate of novel imaging mechanism. It is often eligible to have the capability to make sound properties and mimic blood arrangement for specific applications. In this review, sufficient BMF materials, liquids, and measures are described which have been generated by utilizing diverse operation mechanism and materials that have sculptured a range of biological systems.
  12. Oglat AA, Matjafri MZ, Suardi N, Abdelrahman MA, Oqlat MA, Oqlat AA
    J Med Ultrasound, 2018;26(3):134-142.
    PMID: 30283199 DOI: 10.4103/JMU.JMU_7_18
    BACKGROUND: To examine the blood flow and detection of the issues related to it by medical ultrasound, it is extremely important to have suitable blood mimicking fluid (BMF) to be used in vitro and to have a movable or portable Doppler flow phantom to use it as a standardizing tool. As known, the main drawbacks of the currently commercial BMF used in the research studies are high in cost and the long time needed for preparation, which is at least 5-7 h. Moreover, there are only two common scatter particles using in BMF as suspension materials such as nylon (Orgasol) and polystyrene. Thus, we need to prepare BMF with both a new mixture fluid and new scatter particle to be as a reflecting factor of ultrasonic waves, for evaluating the speed of sound of the blood flow in the same method like in the research study of ultrasound with relatively low-cost and less consuming time of preparation. However, both the acoustical and physical features of the Doppler flow phantom components (BMF and tissue mimicking material) must correspond the features of the human tissues to make the examination significance. In addition, the BMF must also represent the hemodynamic features of real human blood.

    METHODS: In this experiment, a new adequate ternary mixture liquid for preparation of BMF applied and suspended with a new scatter particle material, this scatter particle material called poly (4-methylstyrene), it used to be adequate with the mixture density and for saving neutrally buoyant. This BMF was prepared for use in the test objects or Doppler flow phantom. The poly (4-methylstyrene) particles were applied for suspension in a mixture liquid or fluid based on three items, which were distilled water, propylene glycol (PG), and polyethylene glycol (PEG) (200 Mw). The diameter of poly (4-methylstyrene) particles is 3-8 μm, which determined by specific sieve in a unit of μm, and the density is 1.040 g/ml.

    RESULTS: Speed of sound, viscosity, density, Backscatter power and attenuation features of mixture fluid or liquid which used for preparing a BMF were measured, discussed, and agreed with draft International Electrotechnical Commission values.

    CONCLUSIONS: There are three various types of ternary items of mixture fluid (water, PG, and PEG [200 Mw]), and a new type of scatter particle material poly (4-methylstyrene) was utilized for preparing the BMF. The scatter particles and mixture fluid prepared and measured at a temperature that simulates the body temperature 37°C. Moreover, one of the advantages of this new blood that is being cheaper than the commercially available BMF products because the PG and the polyethylene glycol (200 Mw) are much cheaper and more available than glycerol and the Dextran that used usually. In addition, new BMF needs less time for preparation compared to the commercial one.

  13. Aldalawi AA, Suardi N, Ahmed NM, Al-Farawn MAS, Dheyab MA, Jebur WI, et al.
    J Lasers Med Sci, 2023;14:e28.
    PMID: 37744010 DOI: 10.34172/jlms.2023.28
    Introduction: The depth of laser light penetration into tissue is a critical factor in determining the effectiveness of photodynamic therapy (PDT). However, the optimal laser light penetration depth necessary for achieving maximum therapeutic outcomes in PDT remains unclear. This study aimed to assess the effectiveness of laser light penetration depth at two specific wavelengths, 532 nm and 660 mm. Methods: Chicken and beef of different thicknesses (1, 3, 5, 10, and 20 mm±0.2 mm) were used as in vitro tissue models. The samples were subjected to irradiation by a low-level laser diode of 532 and 660 nm in continuous mode for 10 minutes. with power densities of 167 and 142 J/cm2, respectively. Laser light transmission through the tissue was measured using a power meter. Results: For beef samples, the 660 nm wavelength achieved a maximum transmission intensity of 30.7% at 1 cm thickness, while the 532 nm laser had a transmission intensity of 6.5%. Similarly, in chicken breast samples, the maximum transmission occurred at 1 cm thickness with 68.1% for the 660 nm wavelength and 18.2% for the 532 nm laser. Conclusion: Results consistently demonstrated a significant correlation (P<0.05) between tissue thickness and laser light penetration. Thicker tissues exhibited faster declines in light transmission intensity compared to thinner tissues within 10 minutes. These findings highlight the importance of further research to enhance light delivery in thicker tissues and improve the efficacy of PDT in various medical conditions.
  14. Ang BJ, Suardi N, Ong EBB, Khasim SNH, Gemanam SJ, Mustafa IS, et al.
    PMID: 38592591 DOI: 10.1007/s43630-024-00564-z
    Impedance spectroscopy was employed to assess the electrical properties of yeast following 405 nm laser irradiation, exploring the effects of visible, non-ionizing laser-induced inactivation as a more selective and safer alternative for photoinactivation applications compared to the use of DNA targeting, ionizing UV light. Capacitance and impedance spectra were obtained for yeast suspensions irradiated for 10, 20, 30, and 40 min using 100 and 200 mW laser powers. Noticeable differences in capacitance spectra were observed at lower frequencies (40 Hz to 1 kHz), with a significant increase at 40 min for both laser powers. β-dispersion was evident in the impedance spectra in the frequency range of 10 kHz to 10 MHz. The characteristic frequency of dielectric relaxation steadily shifted to higher frequencies with increasing irradiation time, with a drastic change observed at 40 min for both laser powers. These changes signify a distinct alteration in the physical state of yeast. A yeast spot assay demonstrated a decrease in cell viability with increasing laser irradiation dose. The results indicate a correlation between changes in electrical properties, cell viability, and the efficacy of 405 nm laser-induced inactivation. Impedance spectroscopy is shown to be an efficient, non-destructive, label-free method for monitoring changes in cell viability in photobiological effect studies. The development of impedance spectroscopy-based real-time studies in photoinactivation holds promise for advancing our understanding of light-cell interactions in medical applications.
  15. Oglat AA, Matjafri MZ, Suardi N, Oqlat MA, Abdelrahman MA, Oqlat AA, et al.
    J Med Ultrasound, 2018;26(3):123-127.
    PMID: 30283197 DOI: 10.4103/JMU.JMU_13_17
    The wall-less flow phantoms with recognized acoustic features (attenuation and speed of sound), interior properties, and dimensions of tissue were prepared, calibrated, and characterized of Doppler ultrasound scanning demands tissue-mimicking materials (TMMs). TMM phantoms are commercially available and ready-made for medical ultrasound applications. Furthermore, the commercial TMM phantoms are proper for ultrasound purpose or estimation of diagnostic imaging techniques according to the chemical materials used for its preparation. However, preparing a desirable TMM for wall-less flow phantom using a specific chemical material according to the specific applications is required for different flow. In this review, TMM and wall-less flow phantoms prepared using different chemical materials and methods were described. The chemical materials used in Doppler ultrasound TMM and wall-less flow phantoms fabricated over the previous decades were of high interest in this review.
Related Terms
Filters
Contact Us

Please provide feedback to Administrator (afdal@afpm.org.my)

External Links