Lignocellulose is the most abundant renewable resource naturally available with great potential for bioconversion to value-added bio-products in this case from coconut husk to reducing sugar. This study was to isolate the lignocellulose bioconversion bacteria from cow dung and termite gut for reducing sugar production. CMC and Congo red dye was used as a selective media to screen for cellulose degrading bacteria while alkaline lignin media used to screen for lignin degrading bacteria. Coconut husk was pretreated using alkaline precipitation method. The 16s rRNA sequence analysis, Gram staining, motility test, crystal formation test and penicillin sensitivity test were used to confirm the bacteria identity. The reducing sugars production was monitor using 3, 5-dinitrosalicyclic acid (DNS) method. Bacterial enzymatic hydrolysis were facilitated through fermentation of 5 locally isolated bacteria at 6 shaking regimes which were 0rpm, 50rpm, 100rpm, 150rpm, 200rpm and 250rpm and conditions were fixed at pH7.0 and temperature 25oC. The total of 39 and 28 colonies were isolated from cow dung and termite gut respectively. The isolates T10, T19, T22, T24, C19, and C37 were identified as Bacillus thuringiensis, Enterobacter aerogenes, Bacillus pumilus, Bacillus vireti, Bacillus anthracis, and Bacillus thuringiensis. In PC medium, 2 bacteria strains, T19 and C37 showed the highest concentration of reducing sugar production (after 18 to 20 hours) which was 1.28 g/L at 150rpm (T19) and 1.27 g/L at 200rpm (C37). While in CMC medium, T19 and C37 also showed the highest concentration of reducing sugar production which was 0.72 g/L at 150rpm (T19) and 0.70 g/L at 200rpm (C37). These bacteria demonstrated significant (p < 0.05) to degrade cellulose was shown by measuring the diameter of clearing zone signifying metabolism of cellulose and also the ability to convert both PC and CMC to reducing sugars measurable by DNS method. This study suggests that the PC resulted in higher reducing sugar production compare to CMC at 150rpm and 200rpm by E. aerogenes and B. thuringiensis which these bacteria isolated from cow dung and termite gut. Future studies on metabolic pathway and specific reducing sugar produced may be done using High Performance Liquid Chromatography (HPLC) analysis.