Chitosan is the second most abundant polymer obtained from the byproduct of seafood. Chitosan and its derivatives and chitosan loaded drugs are the recent area of interest against microbial pathogenesis. The cationic chitosan nanoparticles (ChNPs) interact with the anionic surfaces of the microbial cell membrane, which promotes antimicrobial activity. Although, ChNPs are potential against pathogenic microbes, selection of adaptable, suitable and cost effective synthesis method is much important. In the present study, ChNPs were synthesized adopting ionic gelation using sodium tripolyphosphate as a cross linking agent and characterized by FTIR, DLS, SEM and TEM analysis. ChNPs were investigated for antimicrobial activity against bacterial (Escherichia coli and Staphylococcus aureus) and fungal (Candida albicans) pathogens. ChNPs showed bactericidal activity at the lower minimum inhibitory concentration of about 40-80 μg mL-1. Interestingly, ChNPs exhibits biocompatible antioxidant property by inhibiting DPPH free radicals at 76% and also proven to be a potential candidate against the microbial pathogenesis with an inevitable applications in biomedicine.
Tissue engineering plays a vital role in the medical field that addresses the repair, regeneration, and replacement of damaged tissues or organs. The development of drug-eluting electrospun nanofiber composed of biological macromolecules plays a key role in providing localized drug delivery and structural support. This review examines the recent development and impact of electrospun nanofibers in the field of tissue engineering and explores their potential applications. This review also investigates into the fabrication techniques of nanofibers, highlighting the use of biopolymers like collagen and chitosan, chiefly, focuses on understanding the mechanisms of drug-releasing features of these nanofibers. Studies concerning the medical applications of these nanofibers, such as wound healing, skin regeneration, bone tissue engineering, and neural repair, were also reviewed. Beyond the application in tissue regeneration, this review also explores the potential efficacy of nanofibres in cancer therapy, antibacterial activity, enzyme immobilization, and biosensing applications. This study provides an up-to-date critical insight into the applications of electrospun nanofiber application and key scalable production processes, underscoring the potential economic impacts of advanced wound care technologies. While outlining current challenges, this paper also offers future perspectives on the design, application, and potential expansion of drug-eluting electrospun fibers in medical sciences, ultimately showcasing their pivotal role in advancing therapeutic outcomes.