Media components such as wheat flour, MgSO4 and particle size were screened by Placket Burman design (PBD) while the operating range was fixed by one-factor-at-a-time method (OFAT), primarily for the enrichment of cassava peels as animal feed. Optimization of the selected media components was carried out using Face–Centered Central Composite Design (FCCCD) of the Response Surface Methodology (RSM) and the responses were measured in term of protein and lignin contents. Statistical analysis of the result showed that the quadratic term of wheat flour and the interaction between wheat flour and particle size were highly significant (P
Solid state bioconversion (SSB) of Ulva seaweed for protein enrichment was conducted over 7-days using Phanerochaete chrysosporium (P. chrysosporium). One-factor-at-a-time (OFAT) optimization of process parameters revealed 70% for moisture content, 9% for inoculum size and 7% for minerals. Optimum substrate depth in 1 kg reactor was 12 mm and the protein increased from 88.51 mg/g to 107.89 mg/g. Fourier transformation infrared spectroscopy (FTIR) analysis showed modifications in the polysaccharide resulting from SSB. Soluble and reducing sugars concentrations improved by 168.88% (from 33.74±0.51 mg/g to 90.72±1.38 mg/g) and 198.95% (from 0.13±0.05 mg/g to 0.40±0.01 mg/g), while total carbohydrate reduced to 107.15±1.21 mg/g from initial concentration of 109.39±1.33 mg/g.