A HPLC method for the detection and quantification of glibenclamide, from
dissolution studies of glibenclamide tablets (5 mg), was developed. The dissolution
test employed was the basket method, operating at 100 rpm, using 1000ml
phosphate buffer pH 7.4 as the dissolution medium. Elution was performed on LC-
18 reverse phase, SupelcosilTM ODS column (4.6mm x 25cm, 5μm) using a mobile
phase consisting of 0.02M monobasic ammonium phosphate in 60%v/v acetonitrile
in water at a flow rate of 2ml/min, using phenacetin as the internal standard. The
eluent was monitored at 254nm with an UV detector. Retention times of the
glibenclamide and phenacetin peaks were 3.61 minutes and 1.8 minutes respectively.
Gelatine is used as an excipient for various pharmaceutical dosage forms, such as capsule shells (both hard and soft),
tablets, suspensions, emulsions and injections (e.g. plasma expanders). It is also broadly used in various industries
such as food and cosmetics. Gelatine is a biopolymer obtained from discarded or unused materials of bovine, porcine,
ovine, poultry and marine industrial farms. The discarded materials can be the skin, tendons, cartilages, bones and
connective tissues. Gelatine sourced from animals is relatively easy and inexpensive to produce. The potential needs of
gelatine cannot be overemphasised. Rising demands, health concerns and religious issues have heightened the need for
alternative sources of gelatine. This review presents the various industrial uses of gelatine and the latest developments
in producing gelatine from various sources.