MyMedR

Displaying all 3 publications

Abstract:

Sort:

Fulltext A modified method for genomic DNA extraction from the fish intestinal microflora

Han Z, Sun J, Lv A, Sung Y, Sun X, Shi H, et al.

AMB Express, 2018 Apr 02;8(1):52.
PMID: 29610998 DOI: 10.1186/s13568-018-0578-3

A modified genomic DNA extraction method named the combination of lysozyme and ultrasonic lysis (CLU) method was used to analyze the fish intestinal microflora. In this method, the physical disruption and chemical lysis steps were combined, and some parameters in the key steps were adjusted. In addition, the results obtained by this method were compared with the results obtained by the Zirmil-beating cell disruption method and the QIAamp Fast DNA Stool Mini Kit. The OD260/OD280ratio and concentration of the DNA extracted using the CLU method were 2.02 and 282.8 µg/µL, respectively; when the incubation temperatures for lysozyme and RNase were adjusted to 37 °C, those values were 2.08 and 309.8 µg/µL, respectively. On the agarose gel, a major high-intensity, discrete band of more than 10 kb was found for the CLU method. However, the smearing intensity of degraded DNA was lower when the incubation temperatures were 60 °C for lysozyme and 30 °C for RNase than when incubation temperatures of 37 °C for lysozyme and 37 °C for RNase were used. The V3 variable region of the prokaryotic 16S rDNA was amplified, and an approximately 600-bp fragment was observed when the DNA extracted using the CLU method was used as a template. The CLU method is simple and cost effective, and it yields high-quality, unsheared, high-molecular-weight DNA, which is comparable to that obtained with a commercially available kit. The extracted DNA has potential for applications in critical molecular biology techniques.
Transcriptome profiling of immune-responsive genes in the intestine of Cynoglossus semilaevis Günther challenged with Shewanella algae

Han Z, Sun J, Lv A, Xian JA, Sung YY, Sun X, et al.

Fish Shellfish Immunol, 2018 Sep;80:291-301.
PMID: 29886138 DOI: 10.1016/j.fsi.2018.06.007

To better understand gene expression in the intestine after Shewanella algae infection and provide insights into its immune roles in the tongue sole, Cynoglossus semilaevis, sequencing-based high-throughput RNA analysis (RNA-Seq) for the intestines between the control group and 12 h post-injection group was performed. After assembly, there was an average of 23,957,159 raw sequencing reads, and 23,943,491 clean reads were obtained after filtering out low-quality reads. Then, 383 differentially expressed genes (DEGs) in the intestines in response to S. algae infection were identified. Subsequently, gene ontology (GO) enrichment and Kyoto Encyclopedia of Genes and Genomes (KEGG) enrichment analyses of the DEGs were conducted to further explore their functions. Among all of the pathways involved, sixteen pathways were related to the immune system, among which the complement and coagulation cascades pathway was the most prominent for immunity-related DEGs, followed by the leukocyte transendothelial migration pathway. Furthermore, the expression levels of twelve selected DEGs in the immune-related pathways were identified by quantitative real-time polymerase chain reaction, substantiating the reliability and reproducibility of the RNA-Seq results. In summary, this study represents an important genomic resource for understanding the potential immune role of the tongue sole intestine from the perspective of gene expression.
The relationship between personality and short video addiction among college students is mediated by depression and anxiety

Zhang L, Zhuo XF, Xing K, Liu Y, Lu F, Zhang JY, et al.

Front Psychol, 2024;15:1465109.
PMID: 39534468 DOI: 10.3389/fpsyg.2024.1465109

BACKGROUND: Short video addiction (SVA) among college students is influenced by personality traits, namely, neuroticism and agreeableness. However, the role of depression and anxiety as mediators remains unclear.
OBJECTIVE: This study aims to explore the mediating role of comorbid depression and anxiety in the relationship between different dimensions of university students' personalities and SVA.
METHODS: The SPSS PROCESS was utilized to analyze data from 804 university students across seven universities in China.
RESULTS: The findings show that neuroticism, agreeableness, and extraversion in the personalities of Chinese university students are all significantly linked to SVA; neuroticism and agreeableness in the personalities of university students have a greater impact on SVA; both neuroticism and agreeableness can first induce depression and then lead to anxiety and SVA, whereas only agreeableness can first lead to anxiety and then result in depression and SVA.
CONCLUSION: This study uncovers the intricate relationship between personality traits and SVA among college students, emphasizing depression and anxiety as critical chain mediators in this relationship. It reveals that neuroticism and agreeableness significantly influence SVA through specific pathways involving depression and anxiety, indicating that interventions targeting these traits are essential.

Filters

Please provide feedback to Administrator (afdal@afpm.org.my)

External Links