METHOD: Kalanchoe pinnata leaf extracts utilized in beverage production were obtained via 3 different extraction techniques (conventional solvent extraction, supercritical fluid extraction, microwave-assisted extraction).
RESULTS: The highest values on 2,2-diphenyl-1-picrylhydrazyl, ferric reducing antioxidant power, and 2,2'-azino-bis-3-ethylbenzthiazoline-6-sulphonic acid assay were from a beverage prepared with supercritical fluid extract. When the prophylactic aspects of a Kalanchoe pinnata-based beverage were explored against carbon tetrachloride- (CCl4-) and gentamicin-induced hepatotoxic conditions in male Wistar rats, results revealed a reduction in serum aspartate aminotransferase, serum alkaline phosphatase, serum alanine transaminase, and bilirubin levels in rats with CCl4 and gentamicin-induced toxicity. The study also concluded that the administration of a therapeutic beverage significantly improved serum total protein, albumin, and globulin levels in Kalanchoe pinnata-treated rats.
CONCLUSIONS: Our findings support the ameliorative potential of Kalanchoe pinnata against liver diseases.
METHOD: The crude extract was fractionated into hexane, dichloromethane, ethyl acetate, acetone and methanol fractions. Cell viability test was carried out and concentrations that maintained cell viability at 80% and more were selected for further study. Hypertrophic model was simulated by inducing H9c2 cells with Ang II (1 µM) and the antihypertrophic effect was assessed by measuring changes in the cell size.
RESULTS: Ang II significantly doubled the cell size compared to normal control groups. Results showed that the crude extract, its ethyl acetate, acetone, and methanol fractions, and bioactive compounds significantly reduced Ang II-induced cell hypertrophy (p