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  1. Fulltext Combining ability for germination traits in Jatropha curcas L
    Islam AK, Anuar N, Yaakob Z, Ghani JA, Osman M
    ScientificWorldJournal, 2013;2013:935981.
    PMID: 24222756 DOI: 10.1155/2013/935981
    Six parents of Jatropha curcas were crossed in half diallel fashion, and the F 1s were evaluated to determine the combining ability for nine germination parameters. The ratio between general combining ability (GCA) and specific combining ability (SCA) variances indicated preponderance of additive gene action for all the characters except germination percentage, time of 50% germination, seedling length, and seedling vigor index. The parents P 1 and P 2 were the best general combiner for most of the characters studied. The cross P 1 × P 5 was the best specific combiner for speed of emergence, germination percentage, germination energy, germination index, and seedling vigor index, the cross P 2 × P 5 for mean germination time, time of 50% germination, and seedling length, and the cross P 4 × P 5 for number of days to first germination. The germination percentage varied from 58.06 to 92.76% among the parents and 53.43 to 98.96% among the hybrids. The highest germination (98.96%) was observed in hybrid P 2 × P 4, and none of the hybrids or parents showed 100% germination. The highest germination index (GI) and seedling vigor index (SVI) were found in hybrid P 1 × P 5 and P 2 × P 5, respectively. The results of this study provide clue for the improvement of Jatropha variety through breeding program.
    Matched MeSH terms: Germination/genetics*
  2. Overexpression of rice High-affinity Potassium Transporter gene OsHKT1;5 improves salinity and drought tolerance in Arabidopsis
    Ullah MA, Abdullah-Zawawi MR, Razalli II, Sukiran NL, Uddin MI, Zainal Z
    Mol Biol Rep, 2024 Dec 07;52(1):40.
    PMID: 39644345 DOI: 10.1007/s11033-024-10130-6
    BACKGROUND: Rice is subjected to various environmental stresses, resulting in significant production losses. Abiotic stresses, particularly drought and salinity, are the leading causes of plant damage worldwide. The High-affinity Potassium Transporter (HKT) gene family plays an important role in enhancing crop stress tolerance by regulating physiological and enzymatic functions.

    METHODS AND RESULTS: This study investigates the effect of overexpressing the rice HKT1;5 gene in Arabidopsis thaliana on its tolerance to salinity and drought. The OsHKT1;5 gene was introduced into Arabidopsis under the control of 35 S promoter of CaMV via floral dip transformation method. PCR confirmed the integration of the transgene into the Arabidopsis genome, while qPCR analysis showed its expression. Three transgenic lines of OsHKT1;5 were used for stress treatment and phenotypic studies. The overexpressed lines showed considerably higher germination rates, increased leaf counts, greater fresh and dry weights of the roots and shoots, higher chlorophyll contents, longer root lengths, and reduced Na+ levels together with increased K+ ions levels after salt and drought treatments, in comparison to wild-type plants. Furthermore, overexpressed lines exhibited higher antioxidant levels than wild-type plants under salinity and drought conditions. In addition, transgenic lines showed higher expression levels of the OsHKT1;5 gene in both roots and shoots compared to wild-type plants.

    CONCLUSIONS: In conclusion, this study revealed OsHKT1;5 as a promising candidate for enhancing tolerance to salinity and drought stresses in rice, marking a significant step toward developing a new rice variety with improved abiotic stress tolerance.

    Matched MeSH terms: Germination/genetics
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