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  1. Fulltext Isolation of Janthinobacterium lividum from early onset neonatal sepsis patients in Malaysia
    Shinkafi SH, Umar S, Neela VK, Noordin SM, Noordin SA, Hudu SA, et al.
    Afr Health Sci, 2019 Sep;19(3):2378-2389.
    PMID: 32127808 DOI: 10.4314/ahs.v19i3.11
    Background: The term early onset neonatal septicaemia (EONS) refers to invasive bacterial infections that primarily involve the blood stream of neonates during the first 3 days of life. Although early onset neonatal septicaemia is relatively uncommon, it may be associated with case fatality rates of 15-30% and substantial morbidity in surviving infants.

    Objectives: This study describes an unusual septicaemia cases with Janthinobacterium lividum in neonatal Intensive Care Units.

    Methods: Bacterial causes of early onset neonatal sepsis in Kuala Lumpur Hospital Malaysia were investigated using broad range 16S rDNA PCR and sequencing. The bacterial DNA was isolated directly from blood without pre-incubation. All samples collected were equally cultured and incubated in automated BACTEC system.

    Results: Two hundred and fifty two neonates were recruited in this study with mean (SD) gestational age of 35.9. Neonates with J. lividum infection lacked microbiological evidence of septicaemia as their blood culture yielded no bacterial growth. However, the PCR analysis of these samples yielded 1100bp corresponding to bacteria species.

    Conclusion: This study demonstrates the value of PCR in detecting bacteria where special growth requirement is involved.

    Matched MeSH terms: Oxalobacteraceae/isolation & purification*
  2. Fulltext Polyhydroxyalkanoate production by antarctic soil bacteria isolated from Casey Station and Signy Island
    Goh YS, Tan IK
    Microbiol Res, 2012 Apr 20;167(4):211-9.
    PMID: 21945102 DOI: 10.1016/j.micres.2011.08.002
    Polyhydroxyalkanoate (PHA) is a family of biopolymers produced by some bacteria and is accumulated intracellularly as carbon and energy storage material. Fifteen PHA-producing bacterial strains were identified from bacteria isolated from Antarctic soils collected around Casey Station (66°17'S, 110°32'E) and Signy Island (60°45'S, 45°36'W). Screening for PHA production was carried out by incubating the isolates in PHA production medium supplemented with 0.5% (w/v) sodium octanoate or glucose. 16S rRNA gene sequence analysis revealed that the isolated PHA-producing strains were mainly Pseudomonas spp. and a few were Janthinobacterium spp. All the isolated Pseudomonas strains were able to produce medium-chain-length (mcl) PHA using fatty acids as carbon source, while some could also produce mcl-PHA by using glucose. The Janthinobacterium strains could only utilize glucose to produce polyhydroxybutyrate (PHB). A Pseudomonas isolate, UMAB-40, accumulated PHA up to 48% cell dry mass when utilizing fatty acids as carbon source. This high accumulation occurred at between 5°C and 20°C, then decreased with increasing temperatures. Highly unsaturated mcl-PHA was produced by UMAB-40 from glucose. Such characteristics may be associated with the ability of UMAB-40 to survive in the cold.
    Matched MeSH terms: Oxalobacteraceae/isolation & purification*
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