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The complex genome and adaptive evolution of polyploid Chinese pepper (Zanthoxylum armatum and Zanthoxylum bungeanum)

Hu L 1 , Xu Z 1 , Fan R 1 , Wang G 2 , Wang F 2 , Qin X 1 Show all authors , Yan L 1 , Ji X 1 , Meng M 3 , Sim S 4 , Chen W 2 , Hao C 1 , Wang Q 1 , Zhu H 5 , Zhu S 6 , Xu P 3 , Zhao H 7 , Lindsey K 8 , Daniell H 9 , Wendel JF 10 , Jin S 2

Affiliations 

  • 1 Spice and Beverage Research Institute, Chinese Academy of Tropical Agricultural Sciences, Wanning, China
  • 2 National Key Laboratory of Crop Genetic Improvement, Huazhong Agricultural University, Wuhan, China
  • 3 State Key Laboratory of Grassland and Agro-Ecosystems, School of Life Sciences, Lanzhou University, Lanzhou, China
  • 4 Academy of Sciences Malaysia, Kuala Lumpur, Malaysia
  • 5 College of Biology and Agricultural Resources, Huanggang Normal University, Huanggang, Hubei, China
  • 6 Jinjiaohong Spice Research Institute, Jinjiaohong Agricultural Technology Group Corporation, Nanjing, China
  • 7 Hainan Key Laboratory for Biosafety Monitoring and Molecular Breeding in Off-Season Reproduction Regions, Haikou, China
  • 8 Department of Biosciences, Durham University, Durham, UK
  • 9 Department of Biochemistry, School of Dental Medicine, University of Pennsylvania, Philadelphia, Pennsylvania, USA
  • 10 Department Ecology, Evolution, and Organismal Biology, Iowa State University, Ames, Iowa, USA
Plant Biotechnol J, 2023 Jan;21(1):78-96.
PMID: 36117410 DOI: 10.1111/pbi.13926

Abstract

Zanthoxylum armatum and Zanthoxylum bungeanum, known as 'Chinese pepper', are distinguished by their extraordinary complex genomes, phenotypic innovation of adaptive evolution and species-special metabolites. Here, we report reference-grade genomes of Z. armatum and Z. bungeanum. Using high coverage sequence data and comprehensive assembly strategies, we derived 66 pseudochromosomes comprising 33 homologous phased groups of two subgenomes, including autotetraploid Z. armatum. The genomic rearrangements and two whole-genome duplications created large (~4.5 Gb) complex genomes with a high ratio of repetitive sequences (>82%) and high chromosome number (2n = 4x = 132). Further analysis of the high-quality genomes shed lights on the genomic basis of involutional reproduction, allomones biosynthesis and adaptive evolution in Chinese pepper, revealing a high consistent relationship between genomic evolution, environmental factors and phenotypic innovation. Our study provides genomic resources and new insights for investigating diversification and phenotypic innovation in Chinese pepper, with broader implications for the protection of plants under severe environmental changes.

* Title and MeSH Headings from MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.

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