Affiliations 

  • 1 Department of Biomedical Sciences, Faculty of Medicine and Health Science, University Putra Malaysia, 43400 UPM Serdang, Selangor, Malaysia; College of Medical Laboratory Technology, Institute for Medical Research, Jin Pahang, 50588 Kuala Lumpur, Malaysia
  • 2 Department of Biomedical Sciences, Faculty of Medicine and Health Science, University Putra Malaysia, 43400 UPM Serdang, Selangor, Malaysia; Laboratory of Molecular Biomedicine, Institute of Bioscience, 43400 UPM Serdang, Selangor, Malaysia
  • 3 Laboratory of Molecular Biomedicine, Institute of Bioscience, 43400 UPM Serdang, Selangor, Malaysia
  • 4 Department of Microbiology and Pathology, Faculty of Veterinary Medicine, 43400 UPM Serdang, Selangor, Malaysia; UPM-MAKNA Cancer Research Laboratory, University Putra Malaysia, 43400 UPM Serdang, Selangor, Malaysia
Pharmacogn Mag, 2016 Jan;12(Suppl 1):S86-95.
PMID: 27041866 DOI: 10.4103/0973-1296.176107

Abstract

Ethyl acetate and dichloromethane extract of Dillenia suffruticosa (EADS and DCMDS, respectively) can be a potential anticancer agent. The effects of EADS and DCMDS on the growth of HeLa cervical cancer cells and the expression of apoptotic-related proteins had been investigated in vitro. Cytotoxicity of the extracts toward the cells was determined by 5-diphenyltetrazolium bromide assay, the effects on cell cycle progression and the mode of cell death were analyzed by flow cytometry technique, while the effects on apoptotic-related genes and proteins were evaluated by quantitative real-time polymerase chain reaction, and Western blot and enzyme-linked immunosorbent assay, respectively. Treatment with DCMDS inhibited (P < 0.05) proliferation and induced apoptosis in HeLa cells. The expression of cyclin B1 was downregulated that led to G2/M arrest in the cells after treatment with DCMDA. In summary, DCMDS induced apoptosis in HeLa cells via endoplasmic reticulum stress-induced apoptotic pathway and dysregulation of mitochondria. The data suggest the potential application of DCMDS in the treatment of cervical cancer.

* Title and MeSH Headings from MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.

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