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A protocol for assessing the adhesion potential of lactic acid bacteria on various cell types

Jayaram L 1 , Ramu R 2 , Puttegowda D 1 , M K J 3 , Kumari V B C 1 , Goh KW 4

Affiliations 

  • 1 Department of Biotechnology and Bioinformatics, JSS Academy of Higher Education & Research, Mysore, Karnataka 570015, India
  • 2 Department of Biotechnology and Bioinformatics, JSS Academy of Higher Education & Research, Mysore, Karnataka 570015, India. Electronic address: ramith.gowda@gmail.com
  • 3 Department of Pharmacology, JSS Medical College, JSS Academy of Higher Education & Research, Mysore, Karnataka 570015, India
  • 4 Faculty of Data Science and Information Technology, INTI International University, Nilai 71800, Malaysia
STAR Protoc, 2025 Feb 05;6(1):103619.
PMID: 39913288 DOI: 10.1016/j.xpro.2025.103619

Abstract

Lactic acid bacteria (LAB) are a large group produced during carbohydrate fermentation, resulting in lactic acid. Here, we present a protocol for probiotic adhesion and interaction into buccal epithelial cells, chicken epithelial cells, and HT-29 cells, as well as for autoaggregation and coaggregation. We describe steps for visualizing LAB adhesion by imaging cell adhesion using a light microscope. We then detail procedures for performing an adhesion assay. For complete details on the use and execution of this protocol, please refer to Kumari et al.1.

* Title and MeSH Headings from MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.

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