Development and evaluation of a loop-mediated isothermal amplification (LAMP) method for Candida glabrata detection

Yahaya H 1 , Cheah YK 2 , Chee HY 3 , Than LTL 4

Affiliations 

  • 1 Department of Medical Laboratory Science, Faculty of Allied Health Sciences, Bayero University, Kano, Nigeria
  • 2 Department of Biomedical Sciences, Faculty of Medicine and Health Sciences, Universiti Putra Malaysia, Serdang, 43400, Selangor, Malaysia
  • 3 Department of Medical Microbiology, Universiti Putra Malaysia, Serdang, 43400, Selangor, Malaysia
  • 4 Department of Medical Microbiology, Universiti Putra Malaysia, Serdang, 43400, Selangor, Malaysia. leslie@upm.edu.my
Eur J Clin Microbiol Infect Dis, 2024 Nov;43(11):2211-2214.
PMID: 39285105 DOI: 10.1007/s10096-024-04934-4

Abstract

PURPOSE: Loop-mediated isothermal amplification (LAMP) is a simple and rapid nucleic acid method for DNA amplification at a constant temperature. The "gold standard" culture method for yeast detection, has low sensitivity with severe consequences, increasing morbidity and mortality rates. Here, we report the development of a LAMP method for the specific detection of C. glabrata.

METHODOLOGY: The specific LAMP primers for C. glabrata detection were designed and evaluated.

RESULTS: The LAMP assay accurately detected C. glabrata with no cross-reactivity with other Candida species.

CONCLUSION: The developed molecular method would be a promising tool in the management of invasive candidiasis.

* Title and MeSH Headings from MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.

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