Affiliations 

  • 1 School of Biological Sciences, Universiti Sains Malaysia, Pulau Pinang, Malaysia
  • 2 Malaysian Institute of Pharmaceuticals and Nutraceuticals, Jalan Bukit Gambir, Pulau Pinang, Malaysia Department of Biological Sciences, Faculty of Science and Technology, Universiti Malaysia Terengganu, Terengganu, Malaysia
  • 3 School of Biological Sciences, Universiti Sains Malaysia, Pulau Pinang, Malaysia shaida@usm.my
Integr Cancer Ther, 2014 May;13(3):NP1-9.
PMID: 22336595 DOI: 10.1177/1534735411433203

Abstract

Previous cytotoxic (anticancer) evaluations ofElephantopus molliswere mainly focused on its elephantopin derivatives neglecting the combined effect of the phytochemicals in its traditionally used extracts. In this study, the cytotoxic mechanism of its extracts was investigated using methylene blue assay. The cytotoxic screening results revealed the ethyl acetate extract as the most potent extract by displaying prominent dose-dependent and time-dependent growth inhibitions in human liver carcinoma HepG2 cells with the lowest EC50value of 9.38 ± 0.43 µg/mL after 72 hours of treatment. Acute exposure of the HepG2 cells to the ethyl acetate extract produced a significant regulation of caspase-3 with the peak expression at 8 hours of treatment (P< .05). DNA fragmentation indicated by DeadEnd Apoptosis Detection System-labeled nuclei cells confirmed that the extract induced apoptotic cell death through caspase-3-dependent pathway in HepG2 cells.

* Title and MeSH Headings from MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.

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