Evaluation of the Antibacterial Efficacy of Azadirachta Indica, Commiphora Myrrha, Glycyrrhiza Glabra Against Enterococcus Faecalis using Real Time PCR

Anand S; Rajan M; Venkateshbabu N; Kandaswamy D; Shravya Y; Rajeswari K

doi:10.2174/1874210601610010160

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Evaluation of the Antibacterial Efficacy of Azadirachta Indica, Commiphora Myrrha, Glycyrrhiza Glabra Against Enterococcus Faecalis using Real Time PCR

Anand S ¹ , Rajan M ² , Venkateshbabu N ³ , Kandaswamy D ² , Shravya Y ² , Rajeswari K ²

Affiliations

¹ Department of Conservative Dentistry and Endodontics, Penang International Dental College, Jalan Bagan Laur,12000, Butterworth, Penang, Malaysia
² Department of Conservative Dentistry and Endodontics, Faculty of Dental Sciences, Sri Ramachandra University, Chennai, 600 116, India
³ Department of Restorative Dentistry, School of Dentistry, International Medical University, Kuala Lumpur, Malaysia

Open Dent J, 2016;10:160-5.

PMID: 27386000 DOI: 10.2174/1874210601610010160

Abstract

AIM: To compare the antibacterial efficacy of Azadirachta indica (Neem), Commiphora myrrha (Myrrh), Glycyrrhiza glabra (Liquorice) with 2% Chlorhexidine (CHX) against E. faecalis by using Real Time PCR.

MATERIALS AND METHODS: A total of fifty teeth specimens (n=50) were inoculated with E. faecalis for 21 days. Specimens were divided into five groups (Group 1: Myrrh, Group 2: Neem, Group 3: Liquorice, Group 4: 2% CHX and Group 5: Saline (negative control)). The intracanal medicaments were packed inside the tooth. After 5 days, the remaining microbial load was determined by using real time PCR.

RESULTS: Threshold cycle (Ct) values of Myrrh extract, Neem extract, Liquorice Extract, 2% CHX and saline were found to be 30.94, 23.85, 21.38, 30.93 and 17.8 respectively.

CONCLUSION: Myrrh extract showed inhibition of E.faecalis equal to that of 2% CHX followed by Neem, Liquorice and Saline.

* Title and MeSH Headings from MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.