Affiliations 

  • 1 Universiti Putra Malaysia
  • 2 UCSI University
  • 3 Universiti Malaysia Sabah
MyJurnal

Abstract

Algal have attracted attention from biomedical scientists as they are a valuable natural
source of secondary metabolites that exhibit antioxidant activities. In this study, singlefactor
experiments were conducted to investigate the best extraction conditions (ethanol
concentration, solid-to-solvent ratio, extraction temperature and extraction time) in extracting
antioxidant compounds and capacities from four species of seaweeds (Sargassum polycystum,
Eucheuma denticulatum , Kappaphycus alvarezzi variance Buaya and Kappaphycus alvarezzi
variance Giant) from Sabah. Total phenolic content (TPC) and total flavonoid content (TFC)
assays were used to determine the phenolic and flavonoid concentrations, respectively, while
2,2-azinobis-3-ethylbenzothiazoline-6-sulfonic acid (ABTS) and 2,2-diphenyl-1-picylhydrazyl
(DPPH) radical scavenging capacity assays were used to evaluate the antioxidant capacities of
all seaweed extracts. Results showed that extraction parameters had significant effect (p < 0.05)
on the antioxidant compounds and antioxidant capacities of seaweed. Sargassum polycystum
portrayed the most antioxidant compounds (37.41 ± 0.01 mg GAE/g DW and 4.54 ± 0.02 mg
CE/g DW) and capacities (2.00 ± 0.01 μmol TEAC/g DW and 0.84 ± 0.01 μmol TEAC/g DW)
amongst four species of seaweed.