Leachate (liquid pollutant), which is highly contaminated with organic matter and toxic substances is a major
problem that arised from landfill. Biological methods have proven to be effective to remove organic matters that are
abundant in leachate. This study is intended to compare the used of free mycelia and immobilized mycelia of the
white-rot fungi, Ganoderma australe for the removal of landfill leachate organics. The organics fraction of landfill
leachate was measured by biological oxygen demand (BOD5), and chemical oxygen demand (COD). The experiment
revealed that free mycelia of G. australe showed capability in removing leachate BOD5 but not COD. However, the
use of immobilized G. australe displayed the best result in the removal of BOD5 and COD leachate after 4 weeks of
treatment in flasks with 93.09% and 17.84% percentage removal of BOD5 and COD, respectively. Therefore, G.
australe can be considered potentially useful in the treatment of landfill leachate as they can help in removing BOD
and COD due to their biodegradative abilities.