A study on the feasibility of using gel electrophoresis technique in grading the agarwood oil
quality was investigated. Prior to electrophoresis, the emulsified agarwood oil droplets were
screened by a diffuse layer of ions that have equal absolute charge to that of the droplets surface
charge in aqueous phase. The condition was obtained by varying the concentrations of nonionic
surfactant; Tween 80 until the critical aggregation concentration (CAC) value of 0.0167%
(v/v) was achieved. The prepared droplets suspended in the aqueous within nano-metre size and
had ability to migrate through the agarose gel with its own specific electrophoretic mobility.
However, due to the limitation of gel pore size, only large oil droplets (>200 nm) indicated
visible bands. Overall, a novel work for grading the emulsified agarwood oil droplet with its
own electrical properties was feasible.