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A duplex endpoint PCR assay for rapid detection and differentiation of Leptospira strains

Benacer D 1 , Zain SNM 1 , Lewis JW 2 , Khalid MKNM 3 , Thong KL 1

Affiliations 

  • 1 Institute of Biological Sciences, Faculty of Science, University of Malaya, Kuala Lumpur, Malaysia
  • 2 School of Biological Sciences, Royal Holloway, University of London, Egham, Surrey, United Kingdom
  • 3 Bacteriology Unit, Institute for Medical Research, Ministry of Health, Kuala Lumpur, Malaysia
Rev Soc Bras Med Trop, 2017 Mar-Apr;50(2):239-242.
PMID: 28562762 DOI: 10.1590/0037-8682-0364-2016

Abstract

INTRODUCTION:: This study aimed to develop a duplex endpoint PCR assay for rapid detection and differentiation of Leptospira strains.

METHODS:: Primers were designed to target the rrs (LG1/LG2) and ligB (LP1/LP2) genes to confirm the presence of the Leptospira genus and the pathogenic species, respectively.

RESULTS:: The assay showed 100% specificity against 17 Leptospira strains with a limit of detection of 23.1pg/µl of leptospiral DNA and sensitivity of 103 leptospires/ml in both spiked urine and water.

CONCLUSIONS:: Our duplex endpoint PCR assay is suitable for rapid early detection of Leptospira with high sensitivity and specificity.

* Title and MeSH Headings from MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.

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