• 1 Department of General Educational Development (GED), Faculty of Science and Information Technology, Daffodil International University, 102 & 102/1, Shukrabad, Mirpur Road, Dhanmondi, Dhaka, 1207, Bangladesh.
  • 2 School of Chemical Sciences and Food Technology, Faculty of Science and Technology, University Kebangsaan Malaysia, Bangi, 43600 UKM, Selangor D.E., Malaysia
  • 3 Department of Chemistry Education, Faculty of Education, Graduate Program, University Riau, Pekanbaru, Riau, 28131, Indonesia
  • 4 Southeast Asia Disaster Prevention Research Initiative (SEADPRI-UKM), Institute For Environment and Development (LESTARI), University Kebangsaan Malaysia, Bangi, 43600 UKM, Selangor D.E., Malaysia
Nanoscale Res Lett, 2017 Dec;12(1):474.
PMID: 28774152 DOI: 10.1186/s11671-017-2236-0


An ultrasensitive electrochemical biosensor for the determination of pathogenic Vibrio cholerae (V. cholerae) DNA was developed based on polystyrene-co-acrylic acid (PSA) latex nanospheres-gold nanoparticles composite (PSA-AuNPs) DNA carrier matrix. Differential pulse voltammetry (DPV) using an electroactive anthraquninone oligonucleotide label was used for measuring the biosensor response. Loading of gold nanoparticles (AuNPs) on the DNA-latex particle electrode has significantly amplified the faradaic current of DNA hybridisation. Together with the use of a reported probe, the biosensor has demonstrated high sensitivity. The DNA biosensor yielded a reproducible and wide linear response range to target DNA from 1.0 × 10(-21) to 1.0 × 10(-8) M (relative standard deviation, RSD = 4.5%, n = 5) with a limit of detection (LOD) of 1.0 × 10(-21) M (R (2) = 0.99). The biosensor obtained satisfactory recovery values between 91 and 109% (n = 3) for the detection of V. cholerae DNA in spiked samples and could be reused for six consecutive DNA assays with a repeatability RSD value of 5% (n = 5). The electrochemical biosensor response was stable and maintainable at 95% of its original response up to 58 days of storage period.

* Title and MeSH Headings from MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.