Affiliations 

  • 1 Department of General Educational Development (GED), Faculty of Science & Information Technology, Daffodil International University, Dhanmondi, Dhaka, 1207, Bangladesh. mahbub_iuchem@yahoo.com
  • 2 School of Chemical Sciences and Food Technology, Faculty of Science and Technology, Universiti Kebangsaan Malaysia, 43600, UKM Bangi, Selangor Darul Ehsan, Malaysia
  • 3 Freshwater Fisheries Research Division, FRI Glami Lemi, 71650, Jelebu, Titi, Negeri Sembilan Darul Khusus, Malaysia
  • 4 The Department of Chemistry Education, Faculty of Education, Universitas Riau, Pekanbaru, Riau, 28293, Indonesia
  • 5 Southeast Asia Disaster Prevention Research Initiative (SEADPRI-UKM), Institute for Environment and Development (LESTARI), Universiti Kebangsaan Malaysia, 43600, UKM Bangi, Selangor Darul Ehsan, Malaysia
Nanoscale Res Lett, 2017 Aug 10;12(1):484.
PMID: 28798991 DOI: 10.1186/s11671-017-2254-y

Abstract

The present research describes a simple method for the identification of the gender of arowana fish (Scleropages formosus). The DNA biosensor was able to detect specific DNA sequence at extremely low level down to atto M regimes. An electrochemical DNA biosensor based on acrylic microsphere-gold nanoparticle (AcMP-AuNP) hybrid composite was fabricated. Hydrophobic poly(n-butylacrylate-N-acryloxysuccinimide) microspheres were synthesised with a facile and well-established one-step photopolymerization procedure and physically adsorbed on the AuNPs at the surface of a carbon screen printed electrode (SPE). The DNA biosensor was constructed simply by grafting an aminated DNA probe on the succinimide functionalised AcMPs via a strong covalent attachment. DNA hybridisation response was determined by differential pulse voltammetry (DPV) technique using anthraquinone monosulphonic acid redox probe as an electroactive oligonucleotide label (Table 1). A low detection limit at 1.0 × 10(-18) M with a wide linear calibration range of 1.0 × 10(-18) to 1.0 × 10(-8) M (R (2) = 0.99) can be achieved by the proposed DNA biosensor under optimal conditions. Electrochemical detection of arowana DNA can be completed within 1 hour. Due to its small size and light weight, the developed DNA biosensor holds high promise for the development of functional kit for fish culture usage.

* Title and MeSH Headings from MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.