Affiliations 

  • 1 Virology & Plant Molecular Biology Groups, International Center for Genetic Engineering and Biotechnology, Aruna Asaf Ali Road, New Delhi, 110067, India
  • 2 Norwegian University of Life Science, P.O. Box 8146 Dep., 0033, Oslo, Norway
  • 3 Department of Virology, National Institute of Immunology, New Delhi, 110067, India
  • 4 Department of Biochemistry, Faculty of Science, Jamia Hamdard, New Delhi, 110062, India
  • 5 Virology & Plant Molecular Biology Groups, International Center for Genetic Engineering and Biotechnology, Aruna Asaf Ali Road, New Delhi, 110067, India. sunil.lal@monash.edu
Virus Genes, 2018 Apr;54(2):199-214.
PMID: 29218433 DOI: 10.1007/s11262-017-1526-z

Abstract

The infectious salmon anaemia virus (ISAV) is a piscine virus, a member of Orthomyxoviridae family. It encodes at least 10 proteins from eight negative-strand RNA segments. Since ISAV belongs to the same virus family as Influenza A virus, with similarities in protein functions, they may hence be characterised by analogy. Like NS1 protein of Influenza A virus, s8ORF2 of ISAV is implicated in interferon antagonism and RNA-binding functions. In this study, we investigated the role of s8ORF2 in RNAi suppression in a well-established Agrobacterium transient suppression assay in stably silenced transgenic Nicotiana xanthi. In addition, s8ORF2 was identified as a novel interactor with SsMov10, a key molecule responsible for RISC assembly and maturation in the RNAi pathway. This study thus sheds light on a novel route undertaken by viral proteins in promoting viral growth, using the host RNAi machinery.

* Title and MeSH Headings from MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.