The use of electroosmotic is fast becoming a proven technique for manipulating particles in microfluidic
systems. Several approaches were experimented to improve the force and thus the moving particles in
the fluid. This paper will study the effect of microelectrode on the moving particles in latex using a
particle image velocimetry and to test the velocity of particles movement at various frequencies from
10kHz to 500kHz. The result shows the behaviour of latex particles at different frequencies varying
from low frequencies up to high frequencies under AC electrokinetic forces such as dielectrophoresis
(DEP) and AC electroosmosis (ACEO).