Affiliations 

  • 1 Universiti Malaysia Perlis
  • 2 Universiti Putra Malaysia
MyJurnal

Abstract

Normalisation is a process of removing systematic variation that affects measured gene expression levels
in microarray experiment. The purpose is to get a more accurate DNA microarray result by deleting
the systematic errors that may have occurred when making the DNA microarray slid. In this paper,
four normalisation methods of Global, Lowess, Quantile and Print-tip are discussed, tested and their
final results compared in the form of Matrixes and graphs. Ideal and real microarray slides have been
used for this project. It was found that the Print-tip normalisation method showed the closest results to
the real result for an ideal microarray slide and it has a straight median line final graph. The Print-tip
normalisation method uses more than one normalization factor that is divided among intervals which
are dependent on the values of the addition of red and green logarithm.