• 1 Universiti Teknologi MARA


Bismuth oxide nanoparticles (Bi2O3 NPs) have gained a spot in the development of novel molecular
probes for in vivo biomedical imaging. It exists in six polymorphic forms and each of them exerts with
different stabilities according to its synthetisation temperature. The aim of this preliminary study is to
determine effect of different synthetiation temperatures on cellular viability in vitro. One hundred µg/ml
Bi2O3 NPs synthesised at 60, 90 and 120°C were characterised using scanning electron microscope (SEM)
and their cytotoxicity was evaluated using cell viability assay (MTT assay) upon 24 hours exposure to
Chang liver cells. Images captured by SEM showed an average diameter of 300 nm monoclinic-shaped
with high crystalline formation of all three Bi2O3 NPs. MTT assay revealed increase in liver cell viability
as the synthetisation temperature of Bi2O3 NPs increase. The outcomes suggested that synthetisation
temperature of Bi2O3 NPs plays a role in cellular viability, hence predictive to the biocompatibility of
these nanoparticles to be applied as in vivo radiographic contrast medium.