Legionellosis is a respiratory infection caused by gram-negative bacteria known as Legionella. Although there are
many species of Legionella, the majority of all reported cases of legionellosis were caused by Legionella pneumophila.
Investigations of Legionella outbreaks comprised sampling building water systems for the presence of Legionella.
The aim of this study is to determine the isolation techniques with the most optimal yield for detection of Legionella
based on the Australian/New Zealand Standard Waters –Examination for Legionella (AS/NZS 3896:2008). This is an
experimental research, in which, Legionella species will be spiked in a bottle of water and various isolation techniques
will be conducted. As most cases of Legionellosis are caused by Legionella pneumophila group, this group of species
will be used.