• 1 Department of Food Science and Engineering, School of Marine Science , Ningbo University , Ningbo 315211 , China
  • 2 School of Life and Sciences , Shanghai University , Shanghai 200444 , China
  • 3 National Institute for Nutrition and Health , Chinese Center for Disease Control and Prevention , Beijing 100050 , China
  • 4 Department of Bioprocess Technology, Faculty of Biotechnology & Bimolecular Sciences , Universiti Putra Malaysia , 43400 UPM Serdang , Selangor Malaysia
J Agric Food Chem, 2018 Jul 11;66(27):7131-7138.
PMID: 29902005 DOI: 10.1021/acs.jafc.8b01393


Phospholipid composition in the milk fat globule membrane (MFGM) fluctuates during the entire lactation period in order to suit the growing needs of newborn infants. The present study elucidated and relatively quantified phospholipid molecular species extracted from human milk (HM), mature human milk (MHM), and infant formulas (with or without MFGM supplementation) using hydrophilic liquid chromatography-electrospray ionization-ion trap-time of flight-mass spectrometry (HILIC-ESI-IT-TOF-MS) system. Principal component analysis was used to clarify the differences between phospholipid composition in HM, MHM, and infant formulas. HM and MHM contained high concentrations of sphingomyeline (HM: 107.61 μg/mL, MHM: 227.18 μg/mL), phosphatidylcholine (HM: 59.96 μg/mL, MHM: 50.77 μg/mL), and phosphatidylethanolamine (PE) (HM: 25.24 μg/mL, MHM: 31.76 μg/mL). Significant concentrations (<300 ng/mL) of arachidonic, eicosapentanoic, and docosahexanoic acids were found to esterify to PE in HM and MHM. Meanwhile, all infant formulas were found to contain high concentrations of phosphatidic acids indicating the possibility of degradation of the fortified MFGM either during processing or storage of the infant formulas.

* Title and MeSH Headings from MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.