• 1 International Medical University, Kuala Lumpur, Malaysia
  • 2 Division of Clinical Dentistry, School of Dentistry, International Medical University, Kuala Lumpur, Malaysia
  • 3 Discipline of Endodontology, Faculty of Dentistry, The University of Hong Kong, Hong Kong SAR, China
  • 4 Faculty of Biomedical Science, School of Health Sciences, International Medical University, Kuala Lumpur, Malaysia
Aust Endod J, 2019 Aug;45(2):171-176.
PMID: 30230109 DOI: 10.1111/aej.12303


In endodontic infections, inflammatory mediators such as cytokines are released, recruited and retained until the infection is eradicated. Root canal therapy is performed to prevent the spread of infection. The aim of this study was to investigate the effects of root canal debridement (cleaning and shaping) on periapical inflammation by measuring the levels of inflammatory cytokines, Interleukin-8 (IL-8) and Interleukin-10 (IL-10). The study includes twenty patients with pulp necrosis and asymptomatic apical periodontitis. Periradicular sample was collected using paper points before and after root canal debridement. Cytokine levels were determined by Sandwich Enzyme-Linked Immunosorbent Assay (ELISA). Data were analysed using paired t-test (PASW Statistics 18) (P = 0.05). All samples showed the presence of IL-8 and IL-10 prior to root canal debridement. Significantly reduced levels (P < 0.05) of IL-8 and IL-10 were detected after root canal debridement. In conclusion, root canal debridement significantly decreased the levels of the tested pro- and anti-inflammatory cytokine in the periradicular interstitial fluid.

* Title and MeSH Headings from MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.