Pomegranate (Punica granatum L.) is one of the oldest known and nutrient rich edible fruits. Peel and Arils of three available cultivars of this fruit in Bangladesh namely, Bangladesh pomegranate, Indian-Mridula and Egypt pomegranate were analyzed to evaluate their biochemical and bioactive properties. The ash, crude fiber, lipid, moisture, pH, titratable acidity, total soluble solid, total sugar as well as bioactive properties included DPPH radical scavenging activity, total phenolic content (TPC), total flavonoid content (TFC), total tannin content (TTC) and vitamin C were assessed in the study. The results of the analysis showed that the ash, pH, titratable acidity, moisture, total sugar ranged from 0.26-0.93%, 3.54-4.01, 1.40-1.87%, 75.4381.20%, 9.02-10.12 g DE/100g for aril and 1-2.03%, 3.53-4.12, 1.75-1.88%, 71.69-76.65%, 21.14-29.19 g DE/100g for peel respectively. However, the seed of all cultivars contained significantly (p