Crude fat and fatty acids composition were examined in 16 different species of Malaysian freshwater fishes. Nine species of farmed and seven species of wild fishes lipids were extracted using chloroform: methanol sohxlet extraction. The results showed that the crude lipids in muscle of farmed fishes were higher than the wild fishes, ranging from 20 - 50 % (wt/wt) and 4 – 15 % (wt/wt) respectively. Asian red-tail catfish showed highest total lipid among other fishes which was of 51.64 % (wt/wt), followed by red pomfret and yellow-tail catfish which were of 46.2% and 43.2% respectively. Eicosapentaenoic acid, EPA and docosahexaenoic acid, DHA in wild fishes were found higher compared to captive fish. Among the fishes studied, grey feather back contain high in EPA (2.9%) and DHA (12.1%) as well as snakeskin gouramy (EPA; 3.0% and DHA; 12.8%) and tengalan (EPA; 1.1% and DHA; 7.2%). Other fishes, on the other hand contain less percentage in w-3 fatty acids. The diet and the environment where the fishes were found are the main reason for the differences in fish fatty acids content.