Jatropha curcas oil was extracted using n-hexane as solvent in the Soxhlet extraction method. The physicochemical properties of Malaysian Jatropha curcas oil were evaluated. The result showed that the Jatropha seeds consist of 60% (dry w/w) crude oil. The physicochemical properties showed that the seed oil contained low moisture level of 0.02±0.01%, acid value (1.50±0.07%), iodine value (91.70 ± 1.44 mg/g), peroxide value (0.66±0.04 miliequivalence/kg) and saponification value of 208.5±0.47 mg/g respectively. Gas chromatography analysis showed that oleic acid (46.00±0.19%) appears as dominant fatty acid in seed oil followed by linoleic acid (31.96±0.19%) and palmitic acid (13.89±0.06%). High performance liquid chromatography (HPLC) results showed that the dominant triacylglycerols present were PLL (22.00%), POP(16.48%), 000(16.48%), 00L(16.23%) and OLL(13.00%).