Lignocellulosic materials consist of lignin walls and cellulose fibrils that are bounded into lignin matrix preventingenzymatic activities to occur efficiently. Natural microorganisms such as fungi have the ability to break down this matrixand make the lignocellulosic components more accessible to enzymes. We report on the ability of four white rot fungi:Oxyporus latemarginatus, Rigidoporus vinctus, Phanerochaete chrysosporium and Coriolus versicolor, to degrade kenaf biomass. Fungi were inoculated separately onto kenaf medium and weight loss was determined after four weeks ofincubation period. We observed O. latemarginatus as the fastest-growing fungus when compared with the rest and thusrecorded the highest in biomass weight loss (3-fold higher). Filtrates from the fermentation were assayed for ligninase activity. All species produced high levels of lignin peroxidase (LiP), about the same amount of laccase except for P. chrysosporium and very low levels of manganase peroxidase (MnP). When analyzing for cellulase activities, all fourspecies produced similar amounts of endoglucanase, exoglucanase and β-glucosidase. Because of its consistently fastgrowth and high enzymatic activities, O. latemarginatus stands as a superior candidate in biological pretreatment of lignocellulosic biomass.