In this study, zinc oxide (ZnO) nanorod arrays were synthesized using a simple hydrothermal reaction on a ZnO seeds/
ITO substrate and applied for the fabrication of enzymatic glucose sensor. ZnO nanorod matrix provided a favourable
environment for the immobilization of glucose oxidase (GOx) and introduced a shuttling way for electronic communication
between GOx and electrode. The performance of different aspect ratio of ZnO nanorods that was produced by varying
hydrothermal reaction time was studied. The aspect ratio of ZnO influenced the GOx enzyme immobilization. The
morphology and structure of prepared ZnO nanorods were characterized by employing scanning electron microscopy
(SEM), and X-ray powder diffraction (XRD). Electrochemical measurements of the sensor showed a reproducible sensitivity
of 2.06 μA/cm2
mM for ZnO matrix grown for 4 h with the aspect ratio of 8.0.